Microbe Lab

Risk group classification of microorganisms

The great majority of microorganisms are beneficial to man, plants, animals and the environment. These microorganisms that are non-pathogenic in nature have been applied since time immemorial to produce goods and services and products that are of immense benefit to man, animals, plants and the environment. These vast numbers of non-pathogenic microorganisms are harmless to […]

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Laboratory Biosafety, Bioterrorism, Microbe Lab, Safety tips in Microbiology Lab, ,

Nycodenz: application and properties

What is Nycodenz Nycodenz is a non-ionic, triiodinated radiopaque substance used primarily in molecular biology and biochemistry for density gradient centrifugation. Its chemical formula is C_19H_26I_3N_3O_9. Nycodenz creates gradients that are used to separate cells, viruses, organelles, and other biological particles based on their density. Nycodenz is a brand name for a non-ionic, iso-osmotic density

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Environmental & Soil Microbiology, Microbe Lab, Techniques in Microbiology Lab, , , , ,

Practical Tips for Resolving Conflict as a Scientist, Research Leader, Scholar

Conflict Resolution: Practical Tips for STEM Leaders These tips presented here was adapted from the website: www.hellobio.com In the fast-paced world of science research, a busy lab can sometimes be a stressful place to work, and on occasion these stresses can lead to conflict among colleagues. Limited resources, difficult time constraints and the pressure to produce

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Microbe Lab, ,

HOW TO DESIGN PRIMERS FOR YOUR PCR EXPERIMENT

Primers are short stretches of DNA that target unique sequences of a DNA molecule and help identify a unique part of a genome or a gene to be copied further. They are usually 18 to 25 nucleotides long. Primers can be synthesized and used for your PCR (polymerase chain reaction) and other molecular biology experiment

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Biotechnology, Microbe Lab, Molecular Microbiology, Techniques in Microbiology Lab, , , , , , , ,

DIFFERENTIAL COUNT FOR CSF

AIM: To provide information on the different white blood cells (neutrophils, basophils, eosinophils, lymphocytes, monocytes) present in the cerebrospinal fluid (CSF) specimen. MATERIAL/APPARATUS: CSF specimen, methylene blue stain, microscope, glass slide, water, Bunsen burner, inoculating loop, immersion oil. METHOD/PROCEDURE FOR DIFFERENTIAL COUNT OF CSF SAMPLE REPORTING OF THE RESULT: Report the number of the different

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Microbe Lab, Techniques in Microbiology Lab,

HAEMOCYTOMETER CELL COUNT FOR CSF SAMPLE

Cells of mammalian or prokaryotic or eukaryotic cells can be counted in the lab from samples using different kinds of counting chmabers. The neubauer counting chamber is one major type of counting chamber generally used for counting cells, manually in the microbiology laboratory. Improved neubauer counting chamber is a manual chamber or haemocytometer that allows

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Microbe Lab, Techniques in Microbiology Lab, , , , ,

Important techniques in microbiology laboratory

DIRECT PLATE COUNTING Direct plate counting is a microbiological technique used to evaluate the actual bacterial content of a product or specimen; and it gives an estimate of viable or living cells present in a sample. It is usually carried out by plating an aliquot of the sample on solid agar medium that supports the

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Microbe Lab, Techniques in Microbiology Lab

METALLO-β-LACTAMASE (MBL) – detection methods

Metallo-beta-lactamases (MBLs) are beta-lactamase enzymes produced by pathogenic bacteria, and which hydrolyzes the carbapenems (e.g. imipenem, meropenem, and ertapenem) and render the antibiotics ineffective for treatment. They are encoded by genes that have been procured by pathogenic bacteria either by mutation or by horizontal gene transfer from other resistant microbes. MBLs efficiently hydrolyze all beta-lactam

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Antibiotic Resistance / Antimicrobial Resistance (AMR), Antimicrobial Susceptibility Testing (AST) & Antibiogram, Microbe Lab, Pharmaceutical Microbiology, , ,

EXTENDED SPECTRUM β-LACTAMASE (ESBL) – detection methods

Extended spectrum beta-lactamases (ESBLs) are beta-lactamase enzymes that breakdown or hydrolyze broad-spectrum beta-lactam drugs especially the third-generation oxyimino-cephalosporins (e.g. ceftazidime, ceftriaxone and cefotaxime). There is no consensus for the actually definition of ESBLs; but the acronym “ESBL” was originally coined to reflect the expanded substrate spectrum of the earlier beta-lactamase enzymes (e.g. TEM- and SHV-enzymes).

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Antibiotic Resistance / Antimicrobial Resistance (AMR), Antimicrobial Susceptibility Testing (AST) & Antibiogram, Microbe Lab, Pharmaceutical Microbiology, , , , , , ,

The 3 Rs (Reduction, Refinement, Replacement): Guiding Principles in the Use of Animals For Biomedical/Scientific Research

The use of laboratory animals including mice, rats, rabbits and primates for scientific/biomedical research is guided by some principles and ethical guidelines that ensure the optimum care, protection and security of animals used in scientific investigations. Animal research is a very important procedure in biomedical research through which novel drugs are tested and scientific knowledge

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Cell Culture Notes, Laboratory Biosafety, Microbe Lab, Safety tips in Microbiology Lab, Techniques in Microbiology Lab, , ,