Western blotting technique or protein immunoblot is used to identify specific proteins separated according to their sizes in an electrophoresis experiment. Protein immunoblot unlike other blotting techniques (e.g. southern and northern blotting) utilizes specific antibodies to identify the protein of interest. It is the transfer of proteins from gel to a matrix or nitrocellulose membrane. […]
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Biotechnology, Molecular Microbiology
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Northern blotting technique is used to detect specific sequences of ribonucleic acid (RNA). The protocol for performing northern blotting technique is similar to that of southern blotting technique. Northern blotting technique was first described by James Alwine and colleagues in 1977 as a molecular biology technique for identifying specific nucleotide sequence of a piece of
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Biotechnology, Molecular Microbiology
Southern blotting, developed by Sir Edward M. Southern in 1975, is a molecular technique used to detect specific DNA sequences. It involves transferring DNA from a gel to a nitrocellulose membrane, followed by hybridization with radiolabeled probes. This method is pivotal in DNA analysis, forensic science, and paternity testing.
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Biotechnology, Molecular Microbiology
Streptococcus pneumoniae, prevalent in the human upper respiratory tract, causes various infections including pneumonia, often in vulnerable individuals. Its virulence is linked to its polysaccharide capsule, interfering with phagocytosis. Early detection and treatment with appropriate antibiotics and preventive vaccination are vital, especially for high-risk groups like the elderly and immunocompromised individuals.
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Bacteriology
Shigella dysenteriae is a Gram-negative, non-motile bacterium causing bacillary dysentery. It’s transmitted primarily through the fecal-oral route, requiring a low dose to initiate infection. Symptoms include bloody diarrhea and abdominal cramps. Diagnosis involves stool cultures, and treatment may include antibiotics for severe cases. Prevention focuses on hygiene and clean water.
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Bacteriology
Treponema pallidum, a Gram-negative spirochaete, causes syphilis, a contagious STD spread through direct contact, including mother-to-child transmission. The disease progresses through primary, secondary, and tertiary stages without treatment, potentially causing severe complications. Laboratory diagnosis relies on serological tests, as the bacterium cannot be cultured in vitro. Prevention includes safe sexual practices and treating infected individuals.
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Bacteriology
Helicobacter pylori is a spiral-shaped bacterium causing peptic and gastric ulcers, linked to stomach cancer. It survives stomach acidity by producing urease, creating an alkaline environment. Spread via fecal-oral route, it induces inflammation in the gastric mucosa. Diagnosis involves invasive and non-invasive tests, treatment includes antibiotics and protein-pump inhibitors. Prevention focuses on hygiene.
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BacteriologySalmonella Typhi, a Gram-negative rod, causes typhoid fever in humans, transmitted through contaminated food and water. Non-typhoid strains, like S. Typhimurium, cause gastrointestinal infections. Infections primarily spread via the fecal-oral route, and effective prevention includes good hygiene, proper food handling, and clean water. Treatment involves antibiotics and hydration.
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BacteriologyRickettsia prowazekii is an obligate intracellular parasite causing epidemic typhus, primarily transmitted via lice bites. Symptoms include vasculitis, thrombosis, and systemic infections. Diagnosis involves serological tests and cell culture. Treatment includes chloramphenicol and tetracyclines; prevention focuses on vector control and hygiene. RMSF and Q fever are related rickettsial diseases.
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Bacteriology