Culture media

Luria Broth (LB) and Luria Agar (LA) Media

Luria-Bertani (LB) broth is the most widely used medium for the growth of bacteria. It is a standard medium for propagation of Escherichia coli. It can also be used when culturing cells for plasmid preparation. LB is a nutrient-rich microbial broth that contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It is also suitable for non-selective […]

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INCUBATION & STERILIZATION TECHNIQUE

Microorganisms are incubated in the incubator at different temperatures and time interval depending on the oxygen requirement of the organisms amongst other vital conditions required for optimum growth. Incubation technique is a microbiological procedure which is used to maintain microbial cultures of culture media at a particular ambient temperature at different time intervals. Microbial cultures

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CULTURING TECHNIQUE

Culturing technique is used for the propagation of microorganisms in the microbiology laboratory; and it is an important procedure required for studying the morphological characteristics of microbes especially on solid culture media. Microorganisms are cultured in various ways and in various conditions using different growth media (inclusive of solid media or broth/liquid media). Bacterial growth

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Chromatic Vancomycin Resistant Enterococci (VRE) agar

Chromatic VRE is a selective and differential chromogenic medium used for the qualitative and presumptive detection of vancomycin-resistant Enterococci directly from clinical samples. This medium is intended as an aid in the detection of the following bacteria: Enterococcus faecalis and Enterococcus faecium with acquired vancomycin resistance. Chromatic VRE agar is not intended to diagnose infection or

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Culture media, Antimicrobial Susceptibility Testing (AST) & Antibiogram, Pharmaceutical Microbiology, ,

Desoxycholate Citrate Agar (DCA)

Desoxycholate Citrate Agar (DCA) is a selective medium for the isolation of Salmonella and Shigella organisms. It is ideal for the investigation of pathogenic Enterobacteria. The Gram-positive organisms are totally inhibited by the sodium citrate and sodium desoxycholate in the medium. Proteus and coliforms are also highly inhibited. Salmonella typhi, S. paratyphi and Shigella species

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Cetrimide selective agar

Cetrimide selective agar is used for the selective cultivation and isolation of Pseudomonas aeruginosa isolates from clinical and non-clinical  samples. The preparation of cetrimide selective agar is elaborated in this section. P. aeruginosa is a pigment-producing bacterium. The organism produces certain types of pigments including pyocyanin (blue-green pigment), pyomelanin (black pigment) and pyoverdine (yellow-green and fluorescent pigment) [Figure

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Calculation for preparing culture media

In this section, you will learn how to calculate for culture media preparation. Calculations for culture media preparation should not be a stressful activity. However, beginners might find some hurdles along the way. And this section is geared towards helping beginners in the filed of microbiology understand the simple math involved in properly calculating for

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BAIRDPARKER AGAR

Baird–parker agar is a selective medium for the enumeration of Staphylococcus aureus in foods and this was first reported by Baird–Parker. Baird-Parker Agar was developed by Baird Parker in 1962. It is a selective medium for the detection and enumeration of coagulase-positive staphylococci from food samples. Baird-Parker agar is a type of agar used for

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