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Melting curve analysis in Real-time PCR

Melting curve analysis and detection systems Melting curve analysis can only be performed with realtime PCR detection technologies in which the fluorophore remains associated with the amplicon. Amplifications that have used SYBR® Green I or SYBR® GreenER™ dye can be subjected to melting curve analysis. Dual-labeled probe detection systems such as TaqMan® probes are not […]

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Biotechnology, Molecular Microbiology, Techniques in Microbiology LabMelting curve analysis, Melting curve analysis in Real-time PCR, no-template control, NTC, primer dimers, qRT-PCR, real time PCR

Real-time PCR primer design

DrChika

Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that

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Biotechnology, Molecular Microbiology, Techniques in Microbiology Labprimer design, qRT-PCR, REAL-TIME PCR COMPONENTS, Real-time PCR primer design

STEPS INVOLVED IN PERFORMING REAL-TIME PCR

DrChika

Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. By measuring the amount of amplified product at each stage during the PCR cycle, quantification is

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Molecular Microbiology, Techniques in Microbiology Labannealing, denaturation, DNA polymerase, extension, PCR, PCR reaction, primers, qRT-PCR, quantitative reverse transcriptase PCR, real time PCR, RT PCR, steps involved in real time PCR