Sample of my research statement and results below: My Research Goal My research focused on antimicrobial resistance (AMR), specifically on the spread of antibiotic resistance genes (ARGs) in non-hospital environments. My goal is to understand the fate and effect of antibiotic mixtures on soil microbiomes, since the threats and effects of antibiotic mixtures on the […]
What is Nycodenz Nycodenz is a non-ionic, triiodinated radiopaque substance used primarily in molecular biology and biochemistry for density gradient centrifugation. Its chemical formula is C_19H_26I_3N_3O_9. Nycodenz creates gradients that are used to separate cells, viruses, organelles, and other biological particles based on their density. Nycodenz is a brand name for a non-ionic, iso-osmotic density
Nycodenz: application and properties Read More »
Environmental & Soil Microbiology, Microbe Lab, Techniques in Microbiology Lab
Luria-Bertani (LB) broth is the most widely used medium for the growth of bacteria. It is a standard medium for propagation of Escherichia coli. It can also be used when culturing cells for plasmid preparation. LB is a nutrient-rich microbial broth that contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It is also suitable for non-selective
Luria Broth (LB) and Luria Agar (LA) Media Read More »
Culture media, Techniques in Microbiology Lab
Real-time PCR fluorescence detection systems Real-time fluorescent PCR chemistries Many real-time fluorescent PCR chemistries exist, but the most widely used are 5” nuclease assays such as TaqMan®Assays and SYBR® Green dye–based assays (Figure 1). The 5” nuclease assay is named for the 5” nuclease activityassociated with Taq DNA polymerase (Figure 2). The 5” nuclease domain
Real-time PCR fluorescence detection systems Read More »
Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
BaselineThe baseline of the real-time PCR reaction refers to the signal level during the initial cycles of PCR, usually cycles 3 to 15, in which there is little change in fluorescent signal. The low-level signal of the baseline can be equated to the background or the “noise” of the reaction (Figure 1). The baseline in
Real-time PCR analysis technology Read More »
Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that
Real-time PCR primer design Read More »
Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Opportunistic mycoses are fungal infections caused by opportunistic fungi that only affect people with weakened immune system. They do not occur in healthy people. Opportunistic mycoses are fungal infections of the body which occur almost exclusively in debilitated patients whose normal defense mechanisms against infections are impaired. Such patients include those on chemotherapy, cancer patients,
OPPORTUNISTIC MYCOSES Read More »
Mycology
What is a research proposal? A research proposal is simply defined as a planned and written statement to seek approval from a supervisor, an institution or an organization to carry out a particular project, especially with the intent of seeking financial assistance for the funding of the anticipated research work. However, students in the undergraduate
RESEARCH PROPOSAL TIPS FOR GRANTS & PROJECTS Read More »
Careers in Microbiology, Research & Proposal Tips & Scholarships links
DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product.
REAL-TIME PCR COMPONENTS Read More »
Molecular Microbiology, Techniques in Microbiology Lab
Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. By measuring the amount of amplified product at each stage during the PCR cycle, quantification is
STEPS INVOLVED IN PERFORMING REAL-TIME PCR Read More »
Molecular Microbiology, Techniques in Microbiology Lab