Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that […]
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Opportunistic mycoses are fungal infections caused by opportunistic fungi that only affect people with weakened immune system. They do not occur in healthy people. Opportunistic mycoses are fungal infections of the body which occur almost exclusively in debilitated patients whose normal defense mechanisms against infections are impaired. Such patients include those on chemotherapy, cancer patients,
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Mycology
What is a research proposal? A research proposal is simply defined as a planned and written statement to seek approval from a supervisor, an institution or an organization to carry out a particular project, especially with the intent of seeking financial assistance for the funding of the anticipated research work. However, students in the undergraduate
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Careers in Microbiology, Research & Proposal Tips & Scholarships links
DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product.
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Molecular Microbiology, Techniques in Microbiology Lab
Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. By measuring the amount of amplified product at each stage during the PCR cycle, quantification is
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Molecular Microbiology, Techniques in Microbiology Lab
The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or complementary DNA (cDNA) template can be copied, or “amplified”, many thousand- to a million-fold using sequence-specific oligonucleotides (or primers), heat-stable DNA polymerase, and thermal cycling. In traditional (endpoint) PCR, detection and quantification
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Bijou bottles are used for transporting and storing microbial cultures in the microbiology laboratory. They are basically cylindrical small glass bottles fitted with a screw cap, and bijou bottles are generally used as a culture medium container for microorganisms (inclusive of bacteria and fungi). Test tube racks are commonly used in the microbiology laboratories to
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General Microbiology
WATER BATH Water bath is a piece of equipment used to maintain culture media at thermostatically-controlled temperatures. They are generally used to keep water at a constant temperature for incubating samples and other reagents in the microbiology laboratory. Culture media or reagents to be maintained at a particular temperature prior to their usage for further
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General Microbiology
HOT AIR OVEN Hot air oven is used for the sterilization of glassware’s (e.g. conical flasks, beakers, stirring rods, glass Petri plates) and other heat-stable materials. The sterilization of materials in the hot air oven is due to dry heat unlike the autoclave that achieves the same purpose but with moist heat. Hot air oven
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General Microbiology
Conflict Resolution: Practical Tips for STEM Leaders These tips presented here was adapted from the website: www.hellobio.com In the fast-paced world of science research, a busy lab can sometimes be a stressful place to work, and on occasion these stresses can lead to conflict among colleagues. Limited resources, difficult time constraints and the pressure to produce
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Microbe Lab