Techniques in Microbiology Lab Archives - Page 6 of 6

GRAM STAINING TECHNIQUE

Gram staining is a general purpose bacteriological identification technique used in the bacteriology section of the microbiology laboratory to identify and differentiate bacteria into two groups i.e. Gram-positive and Gram-negative. It was discovered by Christian Gram (1853-1938), a Danish scientist in 1884. Christian Gram showed that the cells of some bacteria could be easily decolorized […]

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Microbe Lab, Bacteriology, Techniques in Microbiology Labacetone, crystal violet, ethanol, Gram negative bacteria, Gram negative cell wall, Gram positive bacteria, Gram stain, Gram staining, Gram staining technique, lugols iodine, safranin

LAMINAR FLOW BIOLOGICAL SAFETY CABINET

DrChika

Laminar flow biological safety cabinet is an air filtration system which protects a laboratory worker from microorganisms or contamination by cells being handled within the cabinet. The laminar flow biological safety cabinet also protects the laboratory room or environment where the work is done from potential danger and aerosols emanating from harmful microorganisms. Work done

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Techniques in Microbiology LabBIOLOGICAL SAFETY CABINET, hood, LAMINAR FLOW, LAMINAR FLOW BIOLOGICAL SAFETY CABINET, safety cabinet

ASEPTIC TECHNIQUE

DrChika

Aseptic technique refers to all the quality control and precautionary measures taken by microbiologists in the laboratory in order to ensure that all working apparatuses are germ-free. It involves numerous processes taken to reduce or prevent the contamination of microbial cultures, reagents and culture media. Aseptic techniques also include all other precautionary measures taken to

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Techniques in Microbiology Labaseptic technique, biosafety laminar flow cabinet, bunsen burner, hood

MICROBIAL COUNT: Total Count & Viable Count

DrChika

Viable cell count: Viable cell count gives an estimate of the total number of living cells present in a given volume of a sample. Viable cell count can be determined by automated machines and with the use of counting chambers such as the haemocytometer (Figure 1) in the microbiology laboratory. Haemocytometers are routinely used in

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Microbe Lab, Microbial Physiology & Metabolism, Techniques in Microbiology Labbacterial cell numbers, cell mass, enumerating bacterial cell numbers, enumeration, haemocytometer, hemocytometer, OD, OD value, Optical density, total count, turbidimetry., turbidometry, viable count

SLIDE RACK & STAINING RACK

DrChika

Slide rack is a piece of vessel used for packing and storing clean glass slides after they have been used for staining. After staining, it is important to wash the glass slides with soap/detergents under running water. The washed glass slides are carefully inserted into the slide rack (Figure 1) where the excess water on

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Microbe Lab, Techniques in Microbiology LabSLIDE RACK, staining, STAINING RACK, STAINING TECHNIQUE

PETRI DISHES

DrChika

Petri dishes are shallow glass or plastic plates that are cylindrical in shape, and which are used to cultivate microorganisms in the microbiology laboratory. Glass Petri dishes are heat-stable and can be sterilized in the autoclave and re-used over a long period of time. But plastic Petri dishes (disposable) cannot be re-used or autoclaved because

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Culture media, Microbe Lab, Techniques in Microbiology Labculture, culture media, culture media plate, PETRI DISHES

PREPARATION OF BACTERIAL SMEAR & HEAT FIXING

DrChika

Bacterial smear is defined as a dehydrated or dried preparation of a bacterial suspension (cells) on a clean glass slide. The source of bacteria for the preparation or making of a smear can be from an agar slant, broth (liquid) culture and from a culture plate. A smear is simply a bacterial preparation made on

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Medical Microbiology, Microbe Lab, Techniques in Microbiology Labbacterial smear, heat fixing, smear, staining, STAINING TECHNIQUE

PRECAUTIONARY MEASURES TO OBSERVE WHILE WORKING IN THE MICROBIOLOGY LABORATORY

DrChika

REFERENCES Atlas R.M (2010). Handbook of Microbiological Media. Fourth edition. American Society of Microbiology Press, USA. Balows A, Hausler W, Herrmann K.L, Isenberg H.D and Shadomy H.J (1991). Manual of clinical microbiology. 5th ed. American Society of Microbiology Press, USA. Beers M.H., Porter R.S., Jones T.V., Kaplan J.L and Berkwits M (2006). The Merck Manual

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Introduction to Microscopy and Microscope

DrChika

The history of microbiology will be incomplete without first mentioning the discovery and development of the microscope. Microscopes are indispensable tools for studying microorganisms because without the invention of microscopes man will find it difficult to see microbes (which are microscopic in nature and are too small to be seen with the naked or unaided

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Microbe Lab, Microscopy & Microscope, Techniques in Microbiology Labmagnification, microscope, microscopy

Centrifuge

DrChika

Centrifuge is an instrument is used to separate suspensions or particulate matter in a liquid based on their different sedimentation properties or densities. The centrifuge machine is generally used in the microbiology laboratory to sediment or separate different macromolecules (especially particulate matter) in a solution. Liquid specimens such as urine and blood can be spinned

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Microbe Lab, Techniques in Microbiology Labcentrifugation, centrifuge, sediment, supernantant