Microbe Lab Archives - Page 4 of 5 - #1 Microbiology Resource Hub

GOOD LABORATORY PRACTICE (GLP)

Good laboratory practice (GLP) is simply defined as a laboratory quality measure or protocol that is concerned with the organizational process and the conditions under which laboratory experimentations (inclusive of other non-clinical studies) are planned, performed, monitored, recorded, archived, retrieved and reported. The main goal of GLP in any medical or non-medical laboratory is to […]

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Laboratory Biosafety, Microbe Lab, Safety tips in Microbiology LabGLP, GOOD LABORATORY PRACTICE, Good Laboratory Practice (GLP)

CLASSIFICATION OF MICROORGANISMS BASED ON HAZARDS AND LABORATORY

DrChika

Pathogenic microorganisms inclusive of viruses, bacteria, and fungi portends health challenges to the general public and healthcare workers or laboratory personnel’s working with these organisms if the proper precautionary measures are not followed in the handling of these microbes. The extensive use of these organisms in research settings or hospital laboratories should be done within

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Laboratory Biosafety, Microbe Lab, Safety tips in Microbiology Lab, Techniques in Microbiology Labbiosafety level 1, biosafety level 3, biosafety level 4, CLASSIFICATION OF MICROORGANISMS BASED ON HAZARDS, CLASSIFICATION OF MICROORGANISMS BASED ON HAZARDS AND LABORATORY, CLASSIFICATION OF MICROORGANISMS BASED ON LABORATORY

TYPES OF MICROSCOPES

DrChika

There abound several numbers of microscopes that can be used by a microscopist to view specimens or samples and microorganisms in the laboratory. The choice of the microscope to be used is usually dependent on the task to be performed by the user and, on the type of specimen or microorganism to be investigated. Normally,

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Microbe Lab, Microscopy & Microscope, Techniques in Microbiology Labbright field microscope, confocal microscope, electron microscope, fluorescence microscope, MICROSCOPES, TYPES OF MICROSCOPES

FUNCTIONS OF THE PARTS OF A MICROSCOPE

DrChika

The microscope has various parts that perform specific function; and it is important that scientists and students acquaint themselves of these components and their functions in order to make optimum use of the equipment (Figure 1). An understanding of the different parts of the microscope is vital for the proper use of the apparatus in

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Techniques in Microbiology Lab, Microbe Lab, Microscopy & MicroscopeFUNCTIONS OF THE PARTS OF A MICROSCOPE, microscope, parts of microscope

TRYPAN BLUE & HAEMOCYTOMETER

DrChika

Trypan blue is defined as a dye or stain that enables us to distinguish between living and dead cells. The trypan blue dye passes through the cell membrane of the dead cells; and this allows the dead cell to take up the colour of the dye. This way, the dead cells appear blue under the

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Microbe Lab, Techniques in Microbiology Labcell count, haemocytometer, TRYPAN BLUE, trypan blue dye

P value

DrChika

The phrase “P value” also means ‘calculated probability’. P value is defined as the probability of finding the observed, or more extreme, results when the null hypothesis (H 0) of a study question is true. P value is also described in terms of rejecting the null hypothesis (H0) when it is actually true. The null

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Formulars & Calculations, Microbe Labalternative hypothesis, hypothesis, null hypothesis, P value, significance level

GRAM STAINING TECHNIQUE

DrChika

Gram staining is a general purpose bacteriological identification technique used in the bacteriology section of the microbiology laboratory to identify and differentiate bacteria into two groups i.e. Gram-positive and Gram-negative. It was discovered by Christian Gram (1853-1938), a Danish scientist in 1884. Christian Gram showed that the cells of some bacteria could be easily decolorized

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Microbe Lab, Bacteriology, Techniques in Microbiology Labacetone, crystal violet, ethanol, Gram negative bacteria, Gram negative cell wall, Gram positive bacteria, Gram stain, Gram staining, Gram staining technique, lugols iodine, safranin

MICROBIAL COUNT: Total Count & Viable Count

DrChika

Viable cell count: Viable cell count gives an estimate of the total number of living cells present in a given volume of a sample. Viable cell count can be determined by automated machines and with the use of counting chambers such as the haemocytometer (Figure 1) in the microbiology laboratory. Haemocytometers are routinely used in

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Microbe Lab, Microbial Physiology & Metabolism, Techniques in Microbiology Labbacterial cell numbers, cell mass, enumerating bacterial cell numbers, enumeration, haemocytometer, hemocytometer, OD, OD value, Optical density, total count, turbidimetry., turbidometry, viable count

SLIDE RACK & STAINING RACK

DrChika

Slide rack is a piece of vessel used for packing and storing clean glass slides after they have been used for staining. After staining, it is important to wash the glass slides with soap/detergents under running water. The washed glass slides are carefully inserted into the slide rack (Figure 1) where the excess water on

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Microbe Lab, Techniques in Microbiology LabSLIDE RACK, staining, STAINING RACK, STAINING TECHNIQUE

PETRI DISHES

DrChika

Petri dishes are shallow glass or plastic plates that are cylindrical in shape, and which are used to cultivate microorganisms in the microbiology laboratory. Glass Petri dishes are heat-stable and can be sterilized in the autoclave and re-used over a long period of time. But plastic Petri dishes (disposable) cannot be re-used or autoclaved because

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Culture media, Microbe Lab, Techniques in Microbiology Labculture, culture media, culture media plate, PETRI DISHES