Techniques in Microbiology Lab Archives - Page 3 of 7

GRAM SMEAR OF CEREBROSPINAL FLUID (CSF) SAMPLE

AIM: To detect the presence of pus cells and bacteria in cerebrospinal fluid (CSF) specimen. MATERIAL/APPARATUS: CSF specimen, Gram staining reagents, microscope, glass slide, immersion oil, Bunsen burner. METHOD/PROCEDURE FOR CSF GRAM STAINING REPORTING OF THE RESULT Look for Gram negative intracellular diplococcic, Gram negative rods, Gram positive diplococci, and pus cells and report same. […]

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Microbe Lab, Techniques in Microbiology LabCEREBROSPINAL FLUID, Gram smear, GRAM SMEAR OF CEREBROSPINAL FLUID (CSF) SAMPLE, Gram staining

MICROSCOPY OF CEREBROSPINAL FLUID (CSF)

DrChika

Cerebrospinal fluid (CSF) samples are obtained with extra precautions using a technique called lumbar puncture. Lumbar puncture is defined a medical procedure in which a sterile needle is inserted into the spinal canal (of a patient suspected of having a central nervous system infection) to obtain cerebrospinal fluid sample for microbiological and/or other medical/diagnostic testing.

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Microbe Lab, Techniques in Microbiology LabCEREBROSPINAL FLUID, CSF, lumber puncture, spinal tap

MEMBRANE FILTRATION TECHNIQUE

DrChika

Filtration is simply defined as the separation of particles from fluid or liquids by the use of a porous material that allows the passage of the fluid but stops or holdback unwanted particles from passing through it. Filtration can be used to separate microorganisms from a liquid or gas in which they are dispersed. It

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Environmental & Soil Microbiology, Microbe Lab, Techniques in Microbiology Labfiltration, Membrane filters, MEMBRANE FILTRATION, MEMBRANE FILTRATION TECHNIQUE

CULTURE OF CEREBROSPINAL FLUID (CSF)

DrChika

AIM: To isolate organism from cerebrospinal fluid (CSF) specimen as an aid in the diagnosis of cerebrospinal meningitis and other microbial infection of the CSF. MATERIAL/APPARATUS: Cerebrospinal fluid (CSF) specimen, blood agar (BA), chocolate agar (CA), Bunsen burner, inoculating loop, incubator, anaerobic jar, MacConkey agar (MCA). NOTE: The CSF specimen should be collected only by

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Microbe Lab, Techniques in Microbiology LabCEREBROSPINAL FLUID, culture, CULTURE OF CEREBROSPINAL FLUID (CSF)

MICROSCOPY OF SPUTUM SPECIMEN

DrChika

AIM: To detect the presence of pus cells and predominant bacteria in sputum specimens as an aid in the diagnosis of lower respiratory tract infections like pneumonia or bronchopneumonia. MATERIAL/APPARATUS: Sputum specimen, glass slide, microscope, immersion oil, inoculating loop, Bunsen burner, piece of stick. METHOD/PROCEDURE FOR SPUTUM SAMPLE MICROSCOPY REPORTING OF THE RESULT: Describe and

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Techniques in Microbiology Lab, Microbe Labmicroscopy, MICROSCOPY OF SPUTUM SPECIMEN, SPUTUM, SPUTUM SPECIMEN

ANTIGEN-ANTIBODY REACTION

DrChika

Antigen-antibody reaction is an immunological reaction in which a particular antibody molecule reacts with a specific antigen to form an antigen-antibody complex which is marked for further immunological response by other components of the immune system. It can occur inside a living organism (i.e. in vivo reaction) and it can also occur outside the host

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Microbe Lab, Immunology & Immune System, Techniques in Microbiology Labantibody, antigen, ANTIGEN-ANTIBODY REACTION

ELISA

DrChika

ELISA is the acronym for “enzyme linked immunosorbent assay”. It is an immunoassay or serological test that is used for the quantification or identification of specific antibodies or antigens in biological specimens (e.g. serum or blood). ELISA is mainly based on the principle of enzyme-substrate reaction. In ELISA, an enzyme (e.g. horseradish enzyme and beta-galactosidase

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Immunology & Immune System, Microbe Lab, Techniques in Microbiology Labdirect ELISA, indirect ELISA

STREAKING TECHNIQUE

DrChika

Streaking is a microbiological technique that is used to obtain pure cultures of microorganisms (particularly bacteria) in the laboratory. It usually involves a series of drawing a loop (carrying an inoculum of the test organism or sample) back and forth on dried solid culture media. As the streaking of the microbe on the dried solid

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Bacteriology, Microbe Lab, Techniques in Microbiology Labpour plate method, spread plate method

ISOLATION TECHNIQUE

DrChika

Isolation technique is a microbiology procedure which is used to obtain pure cultures of microorganisms especially from a mixed culture or specimen. It includes culturing microorganisms or specimens on selective media which allow the growth of particular organisms. After culturing, bacterial growth is often expressed on solid culture media as colonies which may contain contaminants

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Microbe Lab, Techniques in Microbiology LabISOLATION, ISOLATION TECHNIQUE, pure cultures

GENOTYPIC DETECTION OF ANTIBIOTIC RESISTANT MICROBES

DrChika

The genotypic detection and characterization of antibiotic resistant genes in pathogenic bacteria is more specific in characterizing resistance genes than the phenotypic detection methods. The term genotypic is derived from genotype – which is the complete genetic makeup of an organism. Genotype describes the overall composition of the genetic information found in an organism. Thus,

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Antimicrobial Susceptibility Testing (AST) & Antibiogram, Pharmaceutical Microbiology, Techniques in Microbiology Lab. Restriction fragment length polymorphism (RFLP), DNA microarray technique, DNA-based methods, Fluorescent in situ hybridization (FISH), GENOTYPIC DETECTION OF ANTIBIOTIC RESISTANT MICROBES, ligase – chain reaction (LCR), nucleotide sequencing, PCR, PCR-single-stranded conformation polymorphism (SSCP), Random Amplified Polymorphic DNA (RAPD)