Isolation technique is a microbiology procedure which is used to obtain pure cultures of microorganisms especially from a mixed culture or specimen. It includes culturing microorganisms or specimens on selective media which allow the growth of particular organisms. After culturing, bacterial growth is often expressed on solid culture media as colonies which may contain contaminants […]
ISOLATION TECHNIQUE Read More »
Microbe Lab, Techniques in Microbiology Lab
Mantoux (Tuberculin) Test is used as a laboratory diagnostic aid to detect reactions of individuals to tuberculin i.e. purified protein derivative (PPD). A tuberculin is any preparation that contains tuberculoprotein – which is usually obtained by the filtration of a culture of tubercle bacilli. PPD is the test antigen in the mantoux skin test. It
MANTOUX (TUBERCULIN) TEST Read More »
Biochemical Tests in Microbiology Lab, Immunology & Immune System
Mycobacterium tuberculosis is a slim, non-motile, non-spore forming, Gram-positive, obligate aerobe, and acid-fast bacillus (rod) with a waxy cell wall. It is found in the genus Mycobacterium and family Mycobacteriaceae. Aside M. tuberculosis, M. bovis (cattle/animal pathogen), M. avium and M. leprae (causative agent of leprosy/Hansen’s disease)are the other important species of the genus Mycobacterium
MYCOBACTERIUM TUBERCULOSIS Read More »
Bacteriology
Haemophilus influenza is a small, Gram-negative, non-sporulating, non-motile, urease positive, indole positive, pleomorphic, rod-like or coccobacillus blood-loving bacterium in the family, Pasteurellaceae. H. influenzae, a non-toxin producing bacterium was first isolated during the 1890 influenza pandemic, and it is often referred to as a “blood-loving” bacterium (i.e. haemophilic bacterium) because it requires growth factors which
HAEMOPHILUS INFLUENZAE Read More »
Bacteriology
Fermentation media is simply defined as those preparations that support the growth of microorganisms used in a fermentation process. The fermentation media used for the operation of fermentation processes in the industry are usually in the liquid phase or solid state. Thus, liquid media (broth) and solid media are basically the major types of media
FERMENTATION MEDIA Read More »
Industrial Microbiology
Beer is an alcoholic beverage that is produced industrially by the microbial fermentation of wort (sugary solutions obtained by the dissolution of malted cereal grains). The process by which wort is converted into alcohol by microbes such as yeasts is known as brewing. The art of beer production (i.e. brewing) is an ancient practice known
THE MICROBIOLOGY OF BEER PRODUCTION Read More »
Industrial Microbiology
Refrigerator is used in the microbiology laboratory to store and preserve laboratory reagents and culture media. Old cultures of microorganisms including new ones are preserved in the refrigerator where the temperature is regulated to maintain the microbial cultures at optimum levels. Also, laboratory refrigerator helps to prevent the contamination of microbial cultures, culture media and
REFRIGERATOR: use and care Read More »
General Microbiology
pH meter is a piece of equipment that is used to maintain the actual pH of a solution i.e. the hydrogen ion concentration of a particular solution. In some instance, a pH indicator is used to evaluate the actual pH of a solution, but the pH of a solution can also be evaluated with the
pH METER: maintenance & care Read More »
General Microbiology
Microscope is a piece of instrument or equipment used by microbiologists to examine microorganisms and objects which are too small to be seen by the naked eye. They are also used by biologists and other scientists because microscope allows investigators to observe objects that are invisible to the naked eye. When materials are examined under
CARE & MAINTENANCE OF THE MICROSCOPE Read More »
General Microbiology
Chemical weighing balances are very sensitive piece of equipments that is used to weigh and measure chemical reagents and materials in the microbiology laboratory. They are analytical apparatuses which play critical role in quantifying materials, and they can be used to measure out or detect the mass of a single particle of chemical substances in the laboratory. Unlike
CHEMICAL WEIGHING BALANCES Read More »
General Microbiology