Molecular Microbiology

STEPS OF PERFORMING GEL ELECTROPHORESIS

Gel electrophoresis is one type of electrophoresis technique, and its procedure is highlighted below. The following materials and steps and steps are employed in gel electrophoresis technique:  In some agarose gel experimentation, ethidium bromide (EtBr) solution is added alongside the DNA solution to be analyzed. However, the EtBr is usually added to the prepared gel […]

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CENTRAL DOGMA (FRAMEWORK) OF MOLECULAR BIOLOGY

The central framework of molecular biology otherwise known as the “central dogma” is the starting point for the actual course of movement of genetic information within the cell’s nucleus of an organism. The transfer of genetic information within the cell of an organism (i.e. in the nucleus) from deoxyribonucleic acid (DNA) to ribonucleic acid (RNA)

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Overview of Molecular Biology

Molecular biology is the study of the genetic makeup of organisms at the level of deoxyribonucleic acid (DNA). It encompasses the study of the ways in which diseases and traits can be passed through the genes especially via from parents to their offspring’s. Genes are the hereditary unit or genetic material that is transmitted from

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GEL ELECTROPHORESIS TECHNIQUE

The term “electrophoresis” refers to the movement of a solid particle (e.g. nucleic acids) through a polymer matrix or gel under the influence of electric field. Electrophoresis is a molecular biology technique that is used to separate nucleic acid molecules and other macromolecules mainly on the basis of their charge to mass ratio as they

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Introduction to Recombinant DNA (rDNA) Technology / Genetic Engineering

Recombinant DNA (rDNA) technology or genetic engineering is the in vitro controlled manipulation of nucleic acids (DNA and RNA) in order to produce new gene products or a combination of genes with improved biological function. It is an advanced and modern form of biotechnology. In genetic engineering, the genetic makeup of living organisms (especially microorganisms)

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