The types of solid support for binding DNA are as follows: 1. Silica Matrix Silica Matrix is a solid support for binding DNA. DNA binds to silica in the presence of high concentrations of chaotropic salts (e.g., guanidium HCL), but protein does not. Salts can be removed with an alcohol-based wash and DNA eluted in […]
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Molecular Microbiology
The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical because it will help the researcher to know the amount of RNA he or she is working with, as well as ascertain its purity and integrity. And in this way,
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Biotechnology uses the tools of molecular biology (i.e. genetic engineering) to produce novel products through the sole manipulation and modification of the genes and gene products of living systems including microorganisms. It borrows scientific expertise from a wide array of disciplines including but not limited to biochemistry, biology, genetics, chemistry, microbiology and molecular biology. Microbial
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Biotechnology, Molecular Microbiology
DNA replication is simply defined as the genetic process of duplicating the genome of a cell. It is an important process that goes on in the cell of every organism each time the organism undergoes reproduction; and DNA replication is a cellular process that occurs in a number of steps. DNA replication generally coordinates the
Gel electrophoresis is one type of electrophoresis technique, and its procedure is highlighted below. The following materials and steps and steps are employed in gel electrophoresis technique: In some agarose gel experimentation, ethidium bromide (EtBr) solution is added alongside the DNA solution to be analyzed. However, the EtBr is usually added to the prepared gel
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Biotechnology, Molecular Microbiology
The central framework of molecular biology otherwise known as the “central dogma” is the starting point for the actual course of movement of genetic information within the cell’s nucleus of an organism. The transfer of genetic information within the cell of an organism (i.e. in the nucleus) from deoxyribonucleic acid (DNA) to ribonucleic acid (RNA)
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Biotechnology, Molecular Microbiology
Genomic DNA is a double helix structure that is composed of several components including purines (adenine and guanine) and pyrimidines (cytosine and thymine) as nitrogenous bases, deoxyribose sugar (a pentose sugar) and phosphate groups. It is a large nucleic acid molecule composed mainly of two polynucleotide chains or strands that are coiled in a complementary
Molecular biology is the study of the genetic makeup of organisms at the level of deoxyribonucleic acid (DNA). It encompasses the study of the ways in which diseases and traits can be passed through the genes especially via from parents to their offspring’s. Genes are the hereditary unit or genetic material that is transmitted from
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Molecular Microbiology
The term “electrophoresis” refers to the movement of a solid particle (e.g. nucleic acids) through a polymer matrix or gel under the influence of electric field. Electrophoresis is a molecular biology technique that is used to separate nucleic acid molecules and other macromolecules mainly on the basis of their charge to mass ratio as they
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Biotechnology, Molecular Microbiology
Recombinant DNA (rDNA) technology or genetic engineering is the in vitro controlled manipulation of nucleic acids (DNA and RNA) in order to produce new gene products or a combination of genes with improved biological function. It is an advanced and modern form of biotechnology. In genetic engineering, the genetic makeup of living organisms (especially microorganisms)
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Biotechnology, Molecular Microbiology