Blotting is used in molecular biology to transfer nucleic acids and proteins from gel to a membrane for identification and analysis. Developed in the 1970s, it combines electrophoresis and immunological methods. There are three main types: Southern (DNA), Northern (RNA), and Western (proteins), each allowing detection and measurement of specific molecules.
Tag: polyacrylamide gel
Ethidium Bromide Staining in Gel Electrophoresis
Ethidium bromide (EtBr) is a fluorescent intercalating agent widely used in molecular biology to visualize […]
GEL ELECTROPHORESIS TECHNIQUE
The term “electrophoresis” refers to the movement of a solid particle (e.g. nucleic acids) through […]