Southern blotting, developed by Sir Edward M. Southern in 1975, is a molecular technique used to detect specific DNA sequences. It involves transferring DNA from a gel to a nitrocellulose membrane, followed by hybridization with radiolabeled probes. This method is pivotal in DNA analysis, forensic science, and paternity testing.
Tag: DNA
WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING
When phenol or a mixture of phenol:chloroform is mixed with a cell Lysate, two phases […]
CENTRAL DOGMA (FRAMEWORK) OF MOLECULAR BIOLOGY
The central framework of molecular biology otherwise known as the “central dogma” is the starting […]
GENOMIC DNA
Genomic DNA is a double helix structure that is composed of several components including purines […]
JAMES WATSON
Born on the 6th of April, 1928, James Watson is an American molecular biologist, geneticist, […]
FRANCIS CRICK (1916-2004)
Francis Crick was an English molecular biologist, biophysicist, and neuroscientist. He co-discovered the molecular structure […]