Techniques in Microbiology Lab Archives - Page 5 of 6

THERMOCYCLER (PCR Machine)

Thermocycler or thermal cycler is a piece of equipment is used for the copying or amplification of specific sequences of nucleotides through a controlled timing and temperature regulation. It is an important tool in the molecular biology laboratory. Thermocycler allows reaction mixtures in specialized tubes known as Eppendorf tubes (PCR tubes) to be heated and […]

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Biotechnology, Molecular Microbiology, Techniques in Microbiology LabPCR, THERMO CYCLER, THERMOCYCLER

TECHNIQUES OF REVIVING MICROBIAL CULTURES

DrChika

The procedures described below are guidelines of the American Type Culture Collection (ATCC). They are specific guidelines that will help you to handle your microbial cells efficiently after procurement, in order to get the best result upon their usage for your experiment. Though the procedure of thawing, reviving or handling microbial cultures may vary from

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Cell Culture Notes, Techniques in Microbiology LabMICROBIAL CULTURES, TECHNIQUES OF REVIREVIVING MICROBIAL CULTURES, TECHNIQUES OF REVIVING MICROBIAL CULTURES

TRYPAN BLUE & HAEMOCYTOMETER

DrChika

Trypan blue is defined as a dye or stain that enables us to distinguish between living and dead cells. The trypan blue dye passes through the cell membrane of the dead cells; and this allows the dead cell to take up the colour of the dye. This way, the dead cells appear blue under the

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Microbe Lab, Techniques in Microbiology Labcell count, haemocytometer, TRYPAN BLUE, trypan blue dye

CEFTAZIDIME-IMIPENEM ANTAGONISM TEST (CIAT)

DrChika

Ceftazidime-imipenem antagonism test (CIAT) is one of the phenotypic confirmation tests that can be used to phenotypically confirm AmpC enzyme production in test bacteria isolates that showed reduced susceptibility or resistance to any of the tested antibiotics used in the screening test for AmpC enzyme production screening. Therefore, AmpC enzyme production in the test organisms

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Pharmaceutical Microbiology, Techniques in Microbiology LabAmpC enzyme, AmpC test, CEFTAZIDIME-IMIPENEM ANTAGONISM TEST, CEFTAZIDIME-IMIPENEM ANTAGONISM TEST (CIAT)

CELL DENSITY METER

DrChika

Cell density meter is an instrument that is used for measuring of the density of (microbial) cells in suspension. The cell density meter is a portable spectrophotometer that can be easily carried from one point to another in the laboratory especially during an experiment. It can be used in the microbiology laboratory to elucidate the

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Techniques in Microbiology LabCELL DENSITY, CELL DENSITY METER, OD value, optical density (OD), spectrophotometer

Glass Plating Beads for spreading bacteria/fungi on culture plate

DrChika

Glass Plating Beads are reusable beads which helps to spread suspensions of microorganisms (bacteria or fungi) in a culture plate even much more than a conventional spreader. They are applicable when turbid suspensions of microbial cells (fungi or bacteria) are to be spread in an culture plate for such purposes as determination of the colony

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Bacteriology, Techniques in Microbiology Labbeads, cell plating, CFU, colony forming unit, colony forming unit (CFU), culture, Glass Plating Beads, Glass Plating Beads for spreading bacteria/fungi on culture plate, rattling platting beads

FTA CARDS

DrChika

The acronym FTA stands for “Flinders Technology Association”. FTA CARDS are chemically treated Whatman filter papers that are designed for the collection, preservation and shipment of biological samples including clinical and environmental samples for subsequent DNA and RNA analysis. FTA cards are cotton-based, cellulose paper containing chemicals that lyse cells or biological samples, denatures proteins,

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Public Health & Parasitic Diseases (Parasitology), Techniques in Microbiology LabDNA analysis, DNA and RNA analysis, Flinders Technology Association, FTA CARDS, RNA analysis

Assessing RNA Purity, Concentration and Integrity

DrChika

The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical because it will help the researcher to know the amount of RNA he or she is working with, as well as ascertain its purity and integrity. And in this way,

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Biotechnology, Molecular Microbiology, Techniques in Microbiology LabAssessing RNA Purity, Concentration and Integrity, RNA purity

INOCULATION TECHNIQUE

DrChika

Inoculation is a microbiology technique which is used to introduce or place specimens and microbial cultures on or into a culture medium. The organism to be transferred into or onto the surface of the culture media (inclusive of solid and liquid or broth media) is known as inoculum. Inoculation is performed in the microbiology laboratory

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Bacteriology, Techniques in Microbiology Labflaming, INOCULATION, INOCULATION TECHNIQUE

BUNSEN BURNER FLAME

DrChika

Bunsen burner is practically a tube or hosepipe that is connected directly to a steady supply of flammable gas contained in a gas cylinder. It is primarily used to achieve aseptic technique in the microbiology laboratory. Usually, a blue flame from the Bunsen burner is used to sterilize the mouth of glass wares, needles, inoculating

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Techniques in Microbiology Labbunsen burner, BUNSEN BURNER FLAME, smear, staining