Real-time PCR is a variation of the standard PCR technique that is commonly used to…
Molecular Biology
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Molecular BiologyNotes on Genetic Engineering & Recombinant DNA TechnologyTechniques in Microbiology Lab
INTRODUCTION TO REAL TIME POLYMERASE CHAIN REACTION (RT PCR)
by DrChikaThe polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology.…
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BiotechnologyMicrobe LabMolecular BiologyNotes on Genetic Engineering & Recombinant DNA TechnologyTechniques in Microbiology Lab
HOW TO DESIGN PRIMERS FOR YOUR PCR EXPERIMENT
by DrChikaPrimers are short stretches of DNA that target unique sequences of a DNA molecule and…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
STEPS INVOLVED IN TRANSFORMING BACTERIAL CELLS
by DrChikaThere are different types of steps involved in the transformation of a bacterial cell in…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
GENE CLONING
by DrChikaCloning is a molecular biology technique which is used to make millions of copies of…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
WESTERN BLOTTING TECHNIQUE
by DrChikaWestern blotting technique or protein immunoblot is used to identify specific proteins separated according to…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
NORTHERN BLOTTING TECHNIQUE
by DrChikaNorthern blotting technique is used to detect specific sequences of ribonucleic acid (RNA). The protocol…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
SOUTHERN BLOTTING TECHNIQUE
by DrChikaSouthern blotting technique is a molecular biology technique in which deoxyribonucleic acid (DNA) is transferred…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
MUTAGENESIS
by DrChikaMutagenesis is the careful alteration of the genetic information of an organism’s genome in such…
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BiotechnologyMolecular BiologyNotes on Genetic Engineering & Recombinant DNA Technology
MUTATION
by DrChikaMutation is a change in the genetic make-up (or DNA) of an organism.It is an…