Bacterial smear is defined as a dehydrated or dried preparation of a bacterial suspension (cells) on a clean glass slide. The source of bacteria for the preparation or making of a smear can be from an agar slant, broth (liquid) culture and from a culture plate. A smear is simply a bacterial preparation made on […]
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Medical Microbiology, Microbe Lab, Techniques in Microbiology Lab
REFERENCES Atlas R.M (2010). Handbook of Microbiological Media. Fourth edition. American Society of Microbiology Press, USA. Balows A, Hausler W, Herrmann K.L, Isenberg H.D and Shadomy H.J (1991). Manual of clinical microbiology. 5th ed. American Society of Microbiology Press, USA. Beers M.H., Porter R.S., Jones T.V., Kaplan J.L and Berkwits M (2006). The Merck Manual
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Microbe Lab, Safety tips in Microbiology Lab, Techniques in Microbiology Lab
Staining is any microbiological process which increases the contrast of organisms when certain dyes or stains are applied to them prior to their examination under the microscope. It is generally the process of colouring specimens and microorganisms so that they can be easily observed and distinguished under the microscope. Staining in the microbiology laboratory is
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Medical Microbiology, Microbe Lab
Inoculating loop or wire loop is a general purpose piece of instrument that allows microbiologist to transfer microorganisms in an aseptic manner from one place to another. Colonies of microbes growing on culture media plates of slants can be transferred onto glass slides or fresh culture medium for further studies, and this is only made
The output of an antimicrobial drug susceptibility test is often expressed as raw data which are either expressed in the form of a zone size/diameter or minimum inhibitory concentration (MIC). The Clinical Laboratory Standard Institute (CLSI) formerly known as National Committee for Clinical Laboratory Standard (NCCLS) has published interpretation criteria for different antimicrobial agents. This
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Antimicrobial Susceptibility Testing (AST) & Antibiogram, Microbe Lab
When a pathogenic microorganism is isolated from the specimen of a patient, the clinical microbiology laboratory will often carry out in vitro antimicrobial susceptibility testing (antibiogram) on the isolated pathogen in order to determine the antimicrobial susceptibility profile of the microbe to a range of antibiotics/drugs using antibiotic susceptibility disks, so that treatment can be
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Antimicrobial Susceptibility Testing (AST) & Antibiogram, Microbe Lab
Chromatic Super CAZ/AVI Chromogenic medium for detecting Ceftazidime-avibactam resistant Gram-negative bacteria. Chromatic Super CAZ/AVI is a selective and differential chromogenic medium used for screening ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa. Chromatic Super CAZ/AVI is not intended to diagnose infection or guide therapy. Results can be interpreted after incubation for 18-24 hours. Subculture to a non-selective
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Antibiotic Resistance / Antimicrobial Resistance (AMR), Antimicrobial Agents & Antibiotics, Antimicrobial Susceptibility Testing (AST) & Antibiogram, Culture media, Microbe Lab, Pharmaceutical Microbiology
Safety first is a general slogan that is obtainable in virtually every spheres of life. It goes to show that we should guarantee safety and the wellbeing of our work environment and those involved in the work so that accident will not occur. When accident occurs (especially when safety rules are not properly imbibed), it
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Microbe Lab, Safety tips in Microbiology Lab
The history of microbiology will be incomplete without first mentioning the discovery and development of the microscope. Microscopes are indispensable tools for studying microorganisms because without the invention of microscopes man will find it difficult to see microbes (which are microscopic in nature and are too small to be seen with the naked or unaided
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Microbe Lab, Microscopy & Microscope, Techniques in Microbiology Lab
Centrifuge is an instrument is used to separate suspensions or particulate matter in a liquid based on their different sedimentation properties or densities. The centrifuge machine is generally used in the microbiology laboratory to sediment or separate different macromolecules (especially particulate matter) in a solution. Liquid specimens such as urine and blood can be spinned
