Nucleotides are nucleosides with one or more phosphate groups covalently attached to the 3′ or […]
Category: Biotechnology
THERMOCYCLER (PCR Machine)
Thermocycler or thermal cycler is a piece of equipment is used for the copying or […]
GENOMIC DNA ISOLATION
The Isolation of Genomic DNA from the bacterium, Escherichia coli is explained in this section […]
WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING
When phenol or a mixture of phenol:chloroform is mixed with a cell Lysate, two phases […]
Assessing RNA Purity, Concentration and Integrity
The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding […]
TOOLS OF BIOTECHNOLOGY
Biotechnology uses the tools of molecular biology (i.e. genetic engineering) to produce novel products through […]
SIGNIFICANCE AND APPLICATIONS OF MICROORGANISMS
Mankind has harnessed the power of microorganisms to produce countless number of products such as […]
DNA REPLICATION
DNA replication is simply defined as the genetic process of duplicating the genome of a […]
FERMENTATION
The phrase fermentation is derived from the Latin word “fevere” – which means “to boil”. […]
STEPS OF PERFORMING GEL ELECTROPHORESIS
Gel electrophoresis is one type of electrophoresis technique, and its procedure is highlighted below. The […]
CHARACTERISTICS OF MICROORGANISMS USED FOR BIOTECHNOLOGICAL PROCESSES
As one of the world’s most research-intensive industry, biotechnology corporations are high-profit making enterprises that […]
CENTRAL DOGMA (FRAMEWORK) OF MOLECULAR BIOLOGY
The central framework of molecular biology otherwise known as the “central dogma” is the starting […]
MICROBIAL METABOLITES: Primary Metabolites & Secondary Metabolites
Microbial metabolites are metabolic intermediate molecules synthesized by microorganisms during and after their growth. They […]
GENOMIC DNA
Genomic DNA is a double helix structure that is composed of several components including purines […]
MICROORGANISMS USED FOR BIOTECHNOLOGICAL PROCESSES
Both prokaryotic and eukaryotic organisms including plants, animals and microorganisms are used for biotechnological manipulations. […]
MICROBIAL GROWTH PHASE
Microbial cells can exhibit different growth patterns depending on the availability of growth nutrients in […]
CHARACTERISTICS OF MICROORGANISMS USED IN INDUSTRIAL MICROBIOLOGY
Microorganisms including bacteria, fungi, actinomycetes and viruses possess some unique qualities different from animals and […]
GEL ELECTROPHORESIS TECHNIQUE
The term “electrophoresis” refers to the movement of a solid particle (e.g. nucleic acids) through […]
Introduction to Recombinant DNA (rDNA) Technology / Genetic Engineering
Recombinant DNA (rDNA) technology or genetic engineering is the in vitro controlled manipulation of nucleic […]
GENERAL SIGNIFICANCE / IMPORTANCE OF FUNGI
References Anaissie E.J, McGinnis M.R, Pfaller M.A (2009). Clinical Mycology. 2nd ed. Philadelphia, PA: Churchill […]
Introduction to Biotechnology
Biotechnology is simply defined as the scientific manipulation of living organisms at the molecular genetics […]