BaselineThe baseline of the real-time PCR reaction refers to the signal level during the initial cycles of PCR, usually cycles 3 to 15, in which there is little change in fluorescent signal. The low-level signal of the baseline can be equated to the background or the “noise” of the reaction (Figure 1). The baseline in […]
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or complementary DNA (cDNA) template can be copied, or “amplified”, many thousand- to a million-fold using sequence-specific oligonucleotides (or primers), heat-stable DNA polymerase, and thermal cycling. In traditional (endpoint) PCR, detection and quantification
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Biotechnology, Molecular Microbiology, Techniques in Microbiology LabPrimers are short stretches of DNA that target unique sequences of a DNA molecule and help identify a unique part of a genome or a gene to be copied further. They are usually 18 to 25 nucleotides long. Primers can be synthesized and used for your PCR (polymerase chain reaction) and other molecular biology experiment
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Biotechnology, Microbe Lab, Molecular Microbiology, Techniques in Microbiology Lab
According to the World Health Organization (WHO) and the Food and Agriculture Organization of the United Nations (FAO), the term “probiotics” refers to live microorganisms (especially bacteria and fungi) that when administered in adequate amounts confer health benefits to the host taking it. The intestinal microbiota is important for the maintenance of good health in
Cell immobilizationis the process of fixing the cells of living organisms including plants, animals, enzymes and microbial cells in a suitable matrix capable of holding them in place. The term “immobilization” is generally used to describe the technique of attaching cells to surfaces either by entrapment or by particle attachment. And the cell immobilization
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Biotechnology
There are different types of steps involved in the transformation of a bacterial cell in gene cloning techniques. Bacterial cells are transformed when they are infused with exogenous DNA from another organism, and this makes them competent enough to express the gene product (or protein) of the gene they are carrying. Usually, the exogenous DNA
STEPS INVOLVED IN TRANSFORMING BACTERIAL CELLS Read More »
Biotechnology, Molecular Microbiology
Cloning is a molecular biology technique which is used to make millions of copies of a particular gene of interest or a piece of DNA molecule. It generally involves taking a piece of DNA molecule (containing specific gene sequence of interest) from a donor organism where it naturally exists and putting same into a cloning
The field of medicine has in the past decades seen the production of many biotherapeutics and/or drugs from genetically modified organisms (GMOs). Many safer and cheaper vaccines and other drugs have been produced from GMOs. For example, injectable insulin (which is used to treat and manage diabetes mellitus disease in diabetic patients) was traditionally produced
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Biotechnology
Genetically modified organisms(GMOs) are living organisms including plants and microorganisms whose genetic material has been artificially manipulated through genetic engineering or recombinant DNA technology in a laboratory. The term GMO also encompasses any living organism that possesses a novel combination of genetic materials obtained from various sources through the use of modern biotechnology. The genetic
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Biotechnology