Microbial Physiology & Metabolism

Mechanisms for uptake of nutrients by microorganisms – diffusion & active transport

Microbial cells employ a variety of mechanisms to uptake nutrients (including macromolecules, inorganic ions, water and other small molecules) from their natural environment or from artificially prepared cultured media which are precisely designed to provide all the essential nutritional requirements and growth factors necessary for microbial or bacterial growth. The cell wall or outer covering

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Microbial Physiology & Metabolism, General Microbiology, , ,

NUTRITIONAL GROUPS OF MICROORGANISMS

Living organisms vary in terms of their mode of nutrition i.e. how they feed or obtain their food. Some organisms are known as autotrophs because they can manufacture their own food; and examples include green plants and green algae. Autotrophs use carbondioxide (CO2) as their sole source of carbon for growth. Plants contain chlorophyll which

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General Microbiology, Microbial Physiology & Metabolism, , , , , ,

Microbial (Bacterial) Nutrition

Bacterial Nutritionis importantfor the optimum growth of the organism because it is the only means by which the organism can acquire all necessary materials required for its cellular biosynthetic activities as well as for the generation of energy (i.e. adenosine triphosphate, ATP). Nutrients are substances required for energy production and other biosynthetic activity necessary for

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Microbial Physiology & Metabolism, General Microbiology, , , , , ,

PROKARYOTIC CELLS

Prokaryotic cells are microbial cells that have chromosomes that are not separated from the cytoplasm by a membrane. Prokaryotic cells are different from eukaryotic cells. They are cells that lack a nucleus and other membrane-enclosed organelles like mitochondrion and chloroplast et cetera. Thus everything inside the cell of a prokaryote is openly accessible within the

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Microbial Physiology & Metabolism, Bacteriology, ,

TYPES OF MICROBIAL CELLS

The cell as we know is the basic unit of life. Microorganisms are cellular entities, and thus could be likened to a cell. They can either exist as unicellular organisms or organisms with multiple cells (i.e. as multicellular organisms). Microbial cells are ubiquitous in the natural environment, and are found in the soil, water, in

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Microbial Physiology & Metabolism, , , ,

MICROBIAL COUNT: Total Count & Viable Count

Viable cell count: Viable cell count gives an estimate of the total number of living cells present in a given volume of a sample. Viable cell count can be determined by automated machines and with the use of counting chambers such as the haemocytometer (Figure 1) in the microbiology laboratory. Haemocytometers are routinely used in

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Microbe Lab, Microbial Physiology & Metabolism, Techniques in Microbiology Lab, , , , , , , , , , , ,

PARTS OF A CELL AND THEIR FUNCTIONS

The cells of all living organisms share several structural characteristics together. Some of the many structural components of a cell (which co-ordinates the cells activities and their functions) are as follows: NUCLEUS Nucleus is the control center of the eukaryotic cell that contains deoxyribonucleic acid (DNA). DNA is the genetic material of the cell which

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