Streaking is a microbiological technique that is used to obtain pure cultures of microorganisms (particularly bacteria) in the laboratory. It usually involves a series of drawing a loop (carrying an inoculum of the test organism or sample) back and forth on dried solid culture media. As the streaking of the microbe on the dried solid […]
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Bacteriology, Microbe Lab, Techniques in Microbiology Lab
Isolation technique is a microbiology procedure which is used to obtain pure cultures of microorganisms especially from a mixed culture or specimen. It includes culturing microorganisms or specimens on selective media which allow the growth of particular organisms. After culturing, bacterial growth is often expressed on solid culture media as colonies which may contain contaminants
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Microbe Lab, Techniques in Microbiology Lab
Helping the physician select the best treatment at a much faster pace based on the right microbial identification and antimicrobial susceptibility test is crucial in any infectious disease management and patient total wellbeing. Clinical microbiology laboratories are the first lines of defense for the detection of antibiotic resistance from clinical pathogens and/or samples. And the
Sputum culture is often recommended in the diagnoses of lower respiratory tract infection (e.g. bacterial pneumonia and pulmonary tuberculosis). Lower respiratory tract infections (LRTIs) are infections/diseases that occur below the voice box (larynx) i.e. in the bronchi and trachea. In this case, an induced or expectorated sputum specimen and not saliva is requested and obtained
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Microbe Lab, Techniques in Microbiology Lab
Urine culture is performed in order to specifically identify organisms that may be causing a urinary tract infection (UTI). Urine in the bladder is often sterile as it is free from microorganisms. It becomes inundated with microorganisms (especially those of the normal micro-flora) when it leaves the bladder. UTI is very common in patients that
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Microbe Lab, Techniques in Microbiology Lab
Blood culture is the most important diagnostic method for detecting and diagnosing bacteraemia (presence of bacteria in blood) and fungimia (i.e. presence of pathogenic fungi in blood) in the clinical microbiology laboratory. Blood specimen required for blood culture technique should be collected from the patient prior to antibiotic therapy in order to increase the sensitivity
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Microbe Lab, Techniques in Microbiology Lab
Stool culture is demanded in the bacteriology laboratory as method for detecting and diagnosing enteric bacterial infections (i.e. infections caused by pathogens in the Enterobacteriaceae family e.g. Salmonella species and Shigella species) that lead to enteric fever, diarrhea and dysentery. Stool culture can also be requested when patients present with other gastrointestinal infections such as
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Microbe Lab, Bacteriology, Techniques in Microbiology Lab
The laboratory diagnosis of fungal infection is mainly based on microscopy and cultural techniques. Several culture media exist for the selective isolation of pathogenic fungi from clinically important specimens. The choice of culture media to be used is largely dependent on the type of mycoses and the category of samples to be analyzed amongst other
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Microbe Lab, Techniques in Microbiology Lab
COLLECTION OF SPUTUM Sputum specimens are collected from patient’s suspected to have respiratory disorders example tuberculosis (TB) and pneumonia. A sterile wide – mouthed container is given to the patient to collect the sputum. Sputum passes via the pharynx and the mouth as it is being collected, and there is always a possibility of being
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Laboratory Biosafety, Microbe Lab, Safety tips in Microbiology Lab
The sample collection unit is one of the most important units in the clinical microbiology laboratory because it acts as a middle man between the patients and the microbiologists working in the laboratory. The sample collection unit is largely responsible for the collection of different clinically important specimens from patients (both outgoing and in-patients), registration
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Microbe Lab, Laboratory Biosafety, Safety tips in Microbiology Lab