Immunology & Immune System

ANTIGEN RETRIEVAL PROTOCOL FOR PARAFFIN-EMBEDDED TISSUE

In the previous section on DEPARAFFINIZATION PRIOR TO IMMUNOHISTOCHEMISTRY STAINING, we looked at deparaffinization and rehydration of paraffin-embedded tissue. In this section, we look at epitope unmasking protocol. Deparaffinization is an important process in the immunohistochemistry staining process; and it must be done prior to immunohistochemistry, especially for paraffin-embedded tissue samples. Deparaffinization is simply defined […]

ANTIGEN RETRIEVAL PROTOCOL FOR PARAFFIN-EMBEDDED TISSUE Read More »

Immunology & Immune System, , , , ,

ANTIBODY SELECTION AND STAINING FOR IMMUNOHISTOCHEMISTRY

We have looked at deparaffinization and rehydration of paraffin-embedded tissue, as well as antigen-retrieval protocol and permeabilization and blocking non-specific binding on the sectioned tissue sample on the slide. In this section, we look briefly on antibody selection and antibody staining for immunohistochemistry. The selection of antibody for immunohistochemistry analysis is important for the overall

ANTIBODY SELECTION AND STAINING FOR IMMUNOHISTOCHEMISTRY Read More »

Immunology & Immune System, , ,

What is a stem cell?

A stem cell is a cell with the unique ability to develop into specialised cell types in the body. In the future they may be used to replace cells and tissues that have been damaged or lost due to disease. Figure 1 is an illustration showing a stem cell giving rise to more stem cells or specialised

What is a stem cell? Read More »

Cell Culture Notes, Immunology & Immune System, , , , , ,

RESPONSES OF THE IMMUNE SYSTEM

The immune system is a complex system which consists of collection of cells, organs, tissues and molecules that work together to protect an organism or animal from disease and invading foreign agent’s (i.e. pathogens). For the immune system to function properly and constantly keep the integrity of the human body intact and free from microbes, it

RESPONSES OF THE IMMUNE SYSTEM Read More »

Immunology & Immune System, , , , ,

HISTORY OF IMMUNOLOGY

Man’s interest in achieving some level of resistance to diseases and their causative agents (i.e. immunity) gave impetus to the study of immunology. The field of immunology actually began when it was observed that people who had earlier suffered and recovered from a particular infection or disease were afterwards discovered to have been protected from

HISTORY OF IMMUNOLOGY Read More »

Immunology & Immune System, ,

Introduction to Immunology / Immune System

Immunology is simply defined as the study of how the immune system of a living organism functions in either a disease condition or a healthy state. It is the study of how an organism responds to foreign bodies (antigens) that it comes in contact with in the environment, and its differentiation from the body’s own

Introduction to Immunology / Immune System Read More »

Immunology & Immune System, , , , , , , ,