The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical because it will help the researcher to know the amount of RNA he or she is working with, as well as ascertain its purity and integrity. And in this way, […]
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab
Biotechnology uses the tools of molecular biology (i.e. genetic engineering) to produce novel products through the sole manipulation and modification of the genes and gene products of living systems including microorganisms. It borrows scientific expertise from a wide array of disciplines including but not limited to biochemistry, biology, genetics, chemistry, microbiology and molecular biology. Microbial
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Biotechnology, Molecular Microbiology
Mankind has harnessed the power of microorganisms to produce countless number of products such as bread, drugs, vaccines, cheese, fuel/energy, probiotics, beer and wine to mention but a few; and many other grounds are being explored and developed using the resourceful nature of microbial cells. Microorganisms are invisible forms of life with tremendous applications in
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General Microbiology, Biotechnology, Industrial Microbiology
DNA replication is simply defined as the genetic process of duplicating the genome of a cell. It is an important process that goes on in the cell of every organism each time the organism undergoes reproduction; and DNA replication is a cellular process that occurs in a number of steps. DNA replication generally coordinates the
The phrase fermentation is derived from the Latin word “fevere” – which means “to boil”. It was first coined and used by Louis Pasteur – who used it to describe the bubbling of sugar solutions especially due to the microbial activity occurring in them. The bubbling action of the sugar (a substrate) was due to
Gel electrophoresis is one type of electrophoresis technique, and its procedure is highlighted below. The following materials and steps and steps are employed in gel electrophoresis technique: In some agarose gel experimentation, ethidium bromide (EtBr) solution is added alongside the DNA solution to be analyzed. However, the EtBr is usually added to the prepared gel
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Biotechnology, Molecular Microbiology
As one of the world’s most research-intensive industry, biotechnology corporations are high-profit making enterprises that seek to increase their economic base while obtaining high returns from their investments. Research and development (R&D) spending in the biotechnology industries of most nations including the United States of America runs into billions of dollars. Recombinant human insulin for
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Biotechnology
The central framework of molecular biology otherwise known as the “central dogma” is the starting point for the actual course of movement of genetic information within the cell’s nucleus of an organism. The transfer of genetic information within the cell of an organism (i.e. in the nucleus) from deoxyribonucleic acid (DNA) to ribonucleic acid (RNA)
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Biotechnology, Molecular Microbiology
Microbial metabolites are metabolic intermediate molecules synthesized by microorganisms during and after their growth. They are the metabolic intermediates of the biosynthetic and degradative pathways of microorganisms Microbes synthesize metabolites or metabolic intermediates at an increasing rate; and these metabolites are usually harvested or recovered by industrial processes usually at the end of the fermentation
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Biotechnology, Industrial Microbiology
Genomic DNA is a double helix structure that is composed of several components including purines (adenine and guanine) and pyrimidines (cytosine and thymine) as nitrogenous bases, deoxyribose sugar (a pentose sugar) and phosphate groups. It is a large nucleic acid molecule composed mainly of two polynucleotide chains or strands that are coiled in a complementary
