Biotechnology Archives - Page 5 of 6 - #1 Microbiology Resource Hub

THERMOCYCLER (PCR Machine)

Thermocycler or thermal cycler is a piece of equipment is used for the copying or amplification of specific sequences of nucleotides through a controlled timing and temperature regulation. It is an important tool in the molecular biology laboratory. Thermocycler allows reaction mixtures in specialized tubes known as Eppendorf tubes (PCR tubes) to be heated and […]

THERMOCYCLER (PCR Machine) Read More »

Biotechnology, Molecular Microbiology, Techniques in Microbiology LabPCR, THERMO CYCLER, THERMOCYCLER

GENOMIC DNA ISOLATION

DrChika

The Isolation of Genomic DNA from the bacterium, Escherichia coli is explained in this section Aim of the experiment: To isolate the genomic DNA from Escherichia coli DH5α cells. PRINCIPLE OF THE EXPERIMENT The isolation and purification of DNA from cells is one of the most common procedures in contemporary molecular biology and embodies a transition

GENOMIC DNA ISOLATION Read More »

Biotechnology, Molecular MicrobiologyDNA isolation, GENOMIC DNA ISOLATION

WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING

DrChika

When phenol or a mixture of phenol:chloroform is mixed with a cell Lysate, two phases form: an aqueous and an organic phase. The polar DNA partitions into the polar or “aqueous phase”, and denatured proteins and other cellular debris partition into the organic phase. Salts are thought to dehydrate proteins, causing them to become less

WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING Read More »

Biotechnology, Molecular MicrobiologyDNA, DNA binding and washing, DNA separation

Assessing RNA Purity, Concentration and Integrity

DrChika

The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical because it will help the researcher to know the amount of RNA he or she is working with, as well as ascertain its purity and integrity. And in this way,

Assessing RNA Purity, Concentration and Integrity Read More »

Biotechnology, Molecular Microbiology, Techniques in Microbiology LabAssessing RNA Purity, Concentration and Integrity, RNA purity

TOOLS OF BIOTECHNOLOGY

DrChika

Biotechnology uses the tools of molecular biology (i.e. genetic engineering) to produce novel products through the sole manipulation and modification of the genes and gene products of living systems including microorganisms. It borrows scientific expertise from a wide array of disciplines including but not limited to biochemistry, biology, genetics, chemistry, microbiology and molecular biology. Microbial

TOOLS OF BIOTECHNOLOGY Read More »

Biotechnology, Molecular Microbiologybioinformatics, biotechnology, DNA sequence analysis, fluxomics, Functional genomics, GenBank, genomics, high-throughput techniques, metabolomics, omics, omics data, protein sequence, protein structure, proteomics, TOOLS OF BIOTECHNOLOGY, transcriptomics

SIGNIFICANCE AND APPLICATIONS OF MICROORGANISMS

DrChika

Mankind has harnessed the power of microorganisms to produce countless number of products such as bread, drugs, vaccines, cheese, fuel/energy, probiotics, beer and wine to mention but a few; and many other grounds are being explored and developed using the resourceful nature of microbial cells. Microorganisms are invisible forms of life with tremendous applications in

SIGNIFICANCE AND APPLICATIONS OF MICROORGANISMS Read More »

General Microbiology, Biotechnology, Industrial Microbiologyimportance of microorganisms, SIGNIFICANCE AND APPLICATIONS OF MICROORGANISMS

DNA REPLICATION

DrChika

DNA replication is simply defined as the genetic process of duplicating the genome of a cell. It is an important process that goes on in the cell of every organism each time the organism undergoes reproduction; and DNA replication is a cellular process that occurs in a number of steps. DNA replication generally coordinates the

DNA REPLICATION Read More »

Biotechnology, Molecular Microbiologydeoxyribose, DNA REPLICATION, ribose, RNA

FERMENTATION

DrChika

The phrase fermentation is derived from the Latin word “fevere” – which means “to boil”. It was first coined and used by Louis Pasteur – who used it to describe the bubbling of sugar solutions especially due to the microbial activity occurring in them. The bubbling action of the sugar (a substrate) was due to

FERMENTATION Read More »

Industrial Microbiology, Biotechnologyferment, fermentation, fermentation process, fermenter, fermentor, fevere, LOUIS PASTEUR

STEPS OF PERFORMING GEL ELECTROPHORESIS

DrChika

Gel electrophoresis is one type of electrophoresis technique, and its procedure is highlighted below. The following materials and steps and steps are employed in gel electrophoresis technique: In some agarose gel experimentation, ethidium bromide (EtBr) solution is added alongside the DNA solution to be analyzed. However, the EtBr is usually added to the prepared gel

STEPS OF PERFORMING GEL ELECTROPHORESIS Read More »

Biotechnology, Molecular Microbiologyagarose gel, electrophoresis, ethidium bromide, gel electrophoresis, STEPS OF PERFORMING GEL ELECTROPHORESIS

CHARACTERISTICS OF MICROORGANISMS USED FOR BIOTECHNOLOGICAL PROCESSES

DrChika

As one of the world’s most research-intensive industry, biotechnology corporations are high-profit making enterprises that seek to increase their economic base while obtaining high returns from their investments. Research and development (R&D) spending in the biotechnology industries of most nations including the United States of America runs into billions of dollars. Recombinant human insulin for

CHARACTERISTICS OF MICROORGANISMS USED FOR BIOTECHNOLOGICAL PROCESSES Read More »

Biotechnologybiotech, biotechnological processes, biotechnology, CHARACTERISTICS OF MICROORGANISMS USED FOR BIOTECHNOLOGICAL PROCESSES