Molecular Microbiology

PROTEIN STRUCTURE – types and function

Proteins are a group of polypeptides that form a molecule of specific biological function; and they comprises mainly of amino acid sequences that are held together by a special type of covalent bonds known as peptide bonds. They are macromolecules that consist of long chains of amino acids held together in structural forms known as […]

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Biotechnology, Molecular Microbiology, ,

THERMOCYCLER (PCR Machine)

Thermocycler or thermal cycler is a piece of equipment is used for the copying or amplification of specific sequences of nucleotides through a controlled timing and temperature regulation. It is an important tool in the molecular biology laboratory. Thermocycler allows reaction mixtures in specialized tubes known as Eppendorf tubes (PCR tubes) to be heated and

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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab, ,

WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING

When phenol or a mixture of phenol:chloroform is mixed with a  cell Lysate, two phases form: an aqueous and an organic phase. The polar DNA partitions into the polar or “aqueous phase”, and denatured proteins and other cellular debris partition into the organic phase. Salts are thought to dehydrate proteins, causing them to become less

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Biotechnology, Molecular Microbiology, ,

TYPES OF SOLID SUPPORT FOR BINDING DNA

The types of solid support for binding DNA are as follows: 1. Silica Matrix Silica Matrix is a solid support for binding DNA. DNA binds to silica in the presence of high concentrations of chaotropic salts (e.g., guanidium HCL), but protein does not. Salts can be removed with an alcohol-based wash and DNA eluted in

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Assessing RNA Purity, Concentration and Integrity

The integrity, purity and concentration of the RNA so isolated should be confirmed before proceeding to the next stage of the experiment. This is critical because it will help the researcher to know the amount of RNA he or she is working with, as well as ascertain its purity and integrity. And in this way,

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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab, ,

TOOLS OF BIOTECHNOLOGY

Biotechnology uses the tools of molecular biology (i.e. genetic engineering) to produce novel products through the sole manipulation and modification of the genes and gene products of living systems including microorganisms. It borrows scientific expertise from a wide array of disciplines including but not limited to biochemistry, biology, genetics, chemistry, microbiology and molecular biology. Microbial

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