Periodontitis is defined as the microbial infection of the periodontal membrane leading to pyorrhea, and resulting in the teeth falling out if left untreated. It is the inflammation of the periodontium. Periodontitis can occur from the penetration of the periodontal membrane by pathogenic bacteria or the activation of resident oral bacteria in the mouth. Prior […]
Gingivitis is simply defined as the inflammation of the gum (gingivae) caused by microbial activity. It is the redness and swelling of the gingival tissues or gum as a result of bacterial infection. Gingivitis is mediated by oral bacteria including Fusobacterium species, Bacteroides, Entamoeba gingivalis, Prevotella species, Bacillus intermedius, Capnocytophaga species, Veillonella speciesand Actinomyces species.
The procedures described below are guidelines of the American Type Culture Collection (ATCC). They are specific guidelines that will help you to handle your microbial cells efficiently after procurement, in order to get the best result upon their usage for your experiment. Though the procedure of thawing, reviving or handling microbial cultures may vary from
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Cell Culture Notes, Techniques in Microbiology Lab
Immunization is the process whereby a person is made immune or resistant to an infectious disease, typically by the administration of a vaccine. Vaccines stimulate the body’s own immune system to protect the person against subsequent infection or disease. Another word that can be used for immunization is vaccination. Immunization is a proven tool for
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Immunology & Immune System, Virology
The Isolation of Genomic DNA from the bacterium, Escherichia coli is explained in this section Aim of the experiment: To isolate the genomic DNA from Escherichia coli DH5α cells. PRINCIPLE OF THE EXPERIMENT The isolation and purification of DNA from cells is one of the most common procedures in contemporary molecular biology and embodies a transition
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Biotechnology, Molecular Microbiology
Level of disease The amount of a particular disease that is usually present in a community is referred to as the baseline or endemic level of the disease. This level is not necessarily the desired level, which may in fact be zero, but rather is the observed level. In the absence of intervention and assuming
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Epidemiology
Desoxycholate Citrate Agar (DCA) is a selective medium for the isolation of Salmonella and Shigella organisms. It is ideal for the investigation of pathogenic Enterobacteria. The Gram-positive organisms are totally inhibited by the sodium citrate and sodium desoxycholate in the medium. Proteus and coliforms are also highly inhibited. Salmonella typhi, S. paratyphi and Shigella species
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Culture media
In the previous section on DEPARAFFINIZATION PRIOR TO IMMUNOHISTOCHEMISTRY STAINING, we looked at deparaffinization and rehydration of paraffin-embedded tissue. In this section, we look at epitope unmasking protocol. Deparaffinization is an important process in the immunohistochemistry staining process; and it must be done prior to immunohistochemistry, especially for paraffin-embedded tissue samples. Deparaffinization is simply defined
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Immunology & Immune System
We have looked at deparaffinization and rehydration of paraffin-embedded tissue, as well as antigen-retrieval protocol and permeabilization and blocking non-specific binding on the sectioned tissue sample on the slide. In this section, we look briefly on antibody selection and antibody staining for immunohistochemistry. The selection of antibody for immunohistochemistry analysis is important for the overall
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Immunology & Immune System
Adenoviruses are found in the viral family known as Adenoviridae. They are non-enveloped viruses with an icosahedral nucleocapsid. Adenoviruses are DNA viruses; and they possess a double stranded DNA molecule. Adenoviruses are a group of viruses that typically cause respiratory illnesses, such as a common cold, conjunctivitis, croup, bronchitis, or pneumonia. In children, adenoviruses usually
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Virology