Cell Culture Notes

BASIS FOR A SUCCESSFUL CELL CULTURE & AREAS THAT ENCOURAGED CELL CULTURE DEVELOPMENT

Having a cell culture without any form of contamination is paramount in the cell culture laboratory. Such success is usually achieved when the laid down principles and aseptic techniques for undertaking a cell culture procedure are conscientiously followed. A successful cell culture technique depends on a number of factors. These factors are: When these factors […]

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Cell Culture Notes

CLASSIFICATION/TYPES OF CELL CULTURE

In the cell culture laboratory, there are many cell culture techniques that are routinely engaged or carried out. These various types of cell culture techniques are shown below: Their only advantage is that they may exhibit some physiological behaviour similar to that obtainable in vivo because they are freshly isolated cells. Primary cell cultures are

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TECHNIQUES OF REVIVING MICROBIAL CULTURES

The procedures described below are guidelines of the American Type Culture Collection (ATCC). They are specific guidelines that will help you to handle your microbial cells efficiently after procurement, in order to get the best result upon their usage for your experiment. Though the procedure of thawing, reviving or handling microbial cultures may vary from

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What is a stem cell?

A stem cell is a cell with the unique ability to develop into specialised cell types in the body. In the future they may be used to replace cells and tissues that have been damaged or lost due to disease. Figure 1 is an illustration showing a stem cell giving rise to more stem cells or specialised

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HISTORICAL PERSPECTIVE OF CELL CULTURE

The history of cell culture cannot be complete without the mention of Henrietta Lacks, whose immortal cell lines (HeLa cells) from cervical carcinoma helped revolutionize the field of cell culture. HeLa cells are immortal cell lines that was derived from a deadly cervical tumor  from a cancer patient (named Henrietta Lacks) in 1951 after she

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REASONS FOR DOING CELL CULTURE

Why is cell, tissue or organ culture embarked upon despite the ethical issues surrounding its development, acceptance and usage? Cell culture has application in a variety of biomedical sciences and even in the industry. It has become one of the major tools used in the life sciences world over. Its application has greatly revolutionized the

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CELLS THAT CAN BE CULTURED IN VITRO USING CELL CULTURE TECHNIQUE

The living cells that are cultured may include: When a whole organ or intact organ fragments of an organism (plant or animal) are removed from an organism and cultured in vitro for the purpose of studying their function and development, the process is called organ culture. The culturing of cells, tissues or organs occurs in

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PHYSIOCHEMICAL ENVIRONMENTAL FACTORS THAT AFFECT CELL CULTURE

Cell or tissue culture experimentations should not be carried out in the regular laboratory space where other laboratory investigations are undertaken. This is critical to avoid contamination of cells in the cell culture plates and also to ensure that all the physiochemical environmental factors that encourage optimal growth of the cells are provided. Thus, cell

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Introduction to Cell Culture

Cell culture is the laboratory technique of growing and maintaining the cells of multicellular organisms (plants and animal cells inclusive) in a favourable artificial environment conducive for growth. It is the maintenance of animal cells (inclusive of plant and human cell lines) in vitro.Such an artificial environment or growth medium mimics the internal environment of

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