Southern blotting, developed by Sir Edward M. Southern in 1975, is a molecular technique used to detect specific DNA sequences. It involves transferring DNA from a gel to a nitrocellulose membrane, followed by hybridization with radiolabeled probes. This method is pivotal in DNA analysis, forensic science, and paternity testing.
Tag: Fluorescence
Ethidium Bromide Staining in Gel Electrophoresis
Ethidium bromide (EtBr) is a fluorescent intercalating agent widely used in molecular biology to visualize […]
Fluorescence microscopy
Fluorescence microscopy is an advanced optical imaging technique that utilizes the phenomenon of fluorescence to […]