BLOTTING

NORTHERN BLOTTING TECHNIQUE

Northern blotting technique is used to detect specific sequences of ribonucleic acid (RNA). The protocol for performing northern blotting technique is similar to that of southern blotting technique. Northern blotting technique was first described by James Alwine and colleagues in 1977 as a molecular biology technique for identifying specific nucleotide sequence of a piece of […]

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Biotechnology, Molecular Microbiology, , , ,

SOUTHERN BLOTTING TECHNIQUE

Southern blotting, developed by Sir Edward M. Southern in 1975, is a molecular technique used to detect specific DNA sequences. It involves transferring DNA from a gel to a nitrocellulose membrane, followed by hybridization with radiolabeled probes. This method is pivotal in DNA analysis, forensic science, and paternity testing.

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Biotechnology, Molecular Microbiology, , , ,