CELLS THAT CAN BE CULTURED IN VITRO USING CELL CULTURE TECHNIQUE

The living cells that are cultured may include:

• Cells – (e.g. blood and microbial cells)

• Tissues – (e.g. skin)

• Organs – (e.g. heart)

When a whole organ or intact organ fragments of an organism (plant or animal) are removed from an organism and cultured in vitro for the purpose of studying their function and development, the process is called organ culture. The culturing of cells, tissues or organs occurs in vitro i.e. in glass or cell culture dishes or flasks; and this is opposed to in vivo processes (i.e. natural processes that occur inside the whole organism from which the cells or tissues where obtained from).

The artificial environment that cell culture provides, and in which cells are engineered to grow in, usually consists of a suitable plastic or glass culture vessel containing liquid or semi-solid medium that supplies all essential growth nutrients for the survival of the cells, tissues or organs being cultured in vitro.

Several sizes of these flasks (usually in the range of 10 – 180 cm²) exist for cell (tissue) culture techniques. While the cap of some of these flasks is vented i.e. some flasks have openings on their caps (Figure 1: Flask B) others are not vented and have no openings on their caps (Figure 1: Flask A). Some tissue (cell) culture flaks are multilayered (e.g. some have up to 5 layers) while others are single layered (Figure 1).

Vented cell/tissue culture flasks provide or allow excess CO₂ generated within the culture flask to exit the flask or to allow CO₂ to enter the flask when incubated inside a CO₂ humidified incubator. Other types of vessels used for cell (tissue) culture apart from the vented and non-vented flasks shown here include the stirrer flasks, multiwall plates, screw-capped vials/phials, Petri dishes and screw-capped conical flasks.  

References

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