Pseudomonas aeruginosa is a non-enteric, oxidase positive, Gram-negative, motile; obligate aerobic straight or curved rod that is found in the genus Pseudomonas and family Pseudomonadaceae. They are recurrently present in small numbers as members of the normal flora in the small intestine and skin of humans especially in hospitalized patients. P. aeruginosa is the most […]
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Bacteriology
Cetrimide selective agar is used for the selective cultivation and isolation of Pseudomonas aeruginosa isolates from clinical and non-clinical samples. The preparation of cetrimide selective agar is elaborated in this section. P. aeruginosa is a pigment-producing bacterium. The organism produces certain types of pigments including pyocyanin (blue-green pigment), pyomelanin (black pigment) and pyoverdine (yellow-green and fluorescent pigment) [Figure
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Culture media
Oxidase test is used to identify microorganisms that produce the enzyme, cytochrome-c oxidase. Cytochrome-c oxidase, a respiratory enzyme is an important enzyme of the electron transport chain (ETC), where it catalyzes the transport of electrons from a donor compound (e.g. NADH) to oxygen, the final electron acceptor. If cytochrome-c oxidase is present, it will oxidize
