culture

CULTURING TECHNIQUE

Culturing technique is used for the propagation of microorganisms in the microbiology laboratory; and it is an important procedure required for studying the morphological characteristics of microbes especially on solid culture media. Microorganisms are cultured in various ways and in various conditions using different growth media (inclusive of solid media or broth/liquid media). Bacterial growth […]

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Bacteriology, Culture media, Techniques in Microbiology Lab, , , ,

CULTURE OF CEREBROSPINAL FLUID (CSF)

AIM: To isolate organism from cerebrospinal fluid (CSF) specimen as an aid in the diagnosis of cerebrospinal meningitis and other microbial infection of the CSF. MATERIAL/APPARATUS: Cerebrospinal fluid (CSF) specimen, blood agar (BA), chocolate agar (CA), Bunsen burner, inoculating loop, incubator, anaerobic jar, MacConkey agar (MCA). NOTE: The CSF specimen should be collected only by

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Microbe Lab, Techniques in Microbiology Lab, ,

Glass Plating Beads for spreading bacteria/fungi on culture plate

Glass Plating Beads are reusable beads which helps to spread suspensions of microorganisms (bacteria or fungi) in a culture plate even much more than a conventional spreader. They are applicable when turbid suspensions of microbial cells (fungi or bacteria) are to be spread in an culture plate for such purposes as determination of the colony

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Bacteriology, Techniques in Microbiology Lab, , , , , , , ,

PETRI DISHES

Petri dishes are shallow glass or plastic plates that are cylindrical in shape, and which are used to cultivate microorganisms in the microbiology laboratory. Glass Petri dishes are heat-stable and can be sterilized in the autoclave and re-used over a long period of time. But plastic Petri dishes (disposable) cannot be re-used or autoclaved because

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Culture media, Microbe Lab, Techniques in Microbiology Lab, , ,

Introduction to Cell Culture

Cell culture is the laboratory technique of growing and maintaining the cells of multicellular organisms (plants and animal cells inclusive) in a favourable artificial environment conducive for growth. It is the maintenance of animal cells (inclusive of plant and human cell lines) in vitro.Such an artificial environment or growth medium mimics the internal environment of

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