CEREBROSPINAL FLUID

GRAM SMEAR OF CEREBROSPINAL FLUID (CSF) SAMPLE

AIM: To detect the presence of pus cells and bacteria in cerebrospinal fluid (CSF) specimen. MATERIAL/APPARATUS: CSF specimen, Gram staining reagents, microscope, glass slide, immersion oil, Bunsen burner. METHOD/PROCEDURE FOR CSF GRAM STAINING REPORTING OF THE RESULT Look for Gram negative intracellular diplococcic, Gram negative rods, Gram positive diplococci, and pus cells and report same. […]

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Microbe Lab, Techniques in Microbiology Lab, , ,

MICROSCOPY OF CEREBROSPINAL FLUID (CSF)

Cerebrospinal fluid (CSF) samples are obtained with extra precautions using a technique called lumbar puncture. Lumbar puncture is defined a medical procedure in which a sterile needle is inserted into the spinal canal (of a patient suspected of having a central nervous system infection) to obtain cerebrospinal fluid sample for microbiological and/or other medical/diagnostic testing. 

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Microbe Lab, Techniques in Microbiology Lab, , ,

CULTURE OF CEREBROSPINAL FLUID (CSF)

AIM: To isolate organism from cerebrospinal fluid (CSF) specimen as an aid in the diagnosis of cerebrospinal meningitis and other microbial infection of the CSF. MATERIAL/APPARATUS: Cerebrospinal fluid (CSF) specimen, blood agar (BA), chocolate agar (CA), Bunsen burner, inoculating loop, incubator, anaerobic jar, MacConkey agar (MCA). NOTE: The CSF specimen should be collected only by

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Microbe Lab, Techniques in Microbiology Lab, ,