Northern blotting technique is used to detect specific sequences of ribonucleic acid (RNA). The protocol for performing northern blotting technique is similar to that of southern blotting technique. Northern blotting technique was first described by James Alwine and colleagues in 1977 as a molecular biology technique for identifying specific nucleotide sequence of a piece of […]
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Biotechnology, Molecular Microbiology
Southern blotting technique is a molecular biology technique in which deoxyribonucleic acid (DNA) is transferred from a gel matrix onto a nitrocellulose matrix or paper. Southern blotting technique was first described by Sir Edward M. Southern in 1975 as a molecular biology technique for identifying specific nucleotide sequence of a piece of DNA. It is
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Biotechnology, Molecular Microbiology
Blotting is the molecular biology technique that allows molecular biologists to transfer the products of a PCR reaction (particularly nucleic acids and protein molecules) from a gel strip onto a matrix or specialized chemically reactive paper for further separation and/or studies. It is the transfer of biological samples from a gel to a membrane and
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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab