Antibiogram

STANDARD QUALITY CONTROL STRAINS FOR ANTIBIOGRAM

Quality control strains (or reference strains)are typed cultures of microorganisms with known antimicrobial susceptibility patterns and which are used to ensure consistency, accuracy and reproducibility of a particular susceptibility test. They are important in any disk diffusion test because susceptibility tests are affected by plethora of factors such as incubation temperature, size of the test […]

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Antimicrobial Susceptibility Testing (AST) & Antibiogram, ,

Antibiogram & Interpretative Standards for Antibiotic (Antibacterial) Disks used for Susceptibility Studies

Antimicrobial Agents /Microorganisms  Disk Code  Disk Potency (µg)   Resistant   Intermediate   Susceptible Amikacin Enterobacteriaceae, P. aeruginosa Acinetobacter spp, Staphylococcus spp     AK 30     ≤14 15-16 ≥17 Amoxycillin-Clavulanic acid EnterobacteriaceaeStaphylococcus spp     Haemophilus spp AMC 20/10 ≤13 ≤19 ≤19   14-17 — —   ≥18 ≥20 ≥20 Ampicillin Enterobacteriaceae and Vibrio cholerae Staphylococcus spp   

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OUTPUT OF ANTIMICROBIAL DRUG SUSCEPTIBILITY TESTING

The output of an antimicrobial drug susceptibility test is often expressed as raw data which are either expressed in the form of a zone size/diameter or minimum inhibitory concentration (MIC). The Clinical Laboratory Standard Institute (CLSI) formerly known as National Committee for Clinical Laboratory Standard (NCCLS) has published interpretation criteria for different antimicrobial agents. This

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Overview of antimicrobial susceptibility testing (AST) / Antibiogram

When a pathogenic microorganism is isolated from the specimen of a patient, the clinical microbiology laboratory will often carry out in vitro antimicrobial susceptibility testing (antibiogram) on the isolated pathogen in order to determine the antimicrobial susceptibility profile of the microbe to a range of antibiotics/drugs using antibiotic susceptibility disks, so that treatment can be

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