southern blotting

SOUTHERN BLOTTING TECHNIQUE

Southern blotting technique is a molecular biology technique in which deoxyribonucleic acid (DNA) is transferred from a gel matrix onto a nitrocellulose matrix or paper. Southern blotting technique was first described by Sir Edward M. Southern in 1975 as a molecular biology technique for identifying specific nucleotide sequence of a piece of DNA. It is […]

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Biotechnology, Molecular Microbiology, , , ,

BLOTTING TECHNIQUE

Blotting is the molecular biology technique that allows molecular biologists to transfer the products of a PCR reaction (particularly nucleic acids and protein molecules) from a gel strip onto a matrix or specialized chemically reactive paper for further separation and/or studies. It is the transfer of biological samples from a gel to a membrane and

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Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab, , , ,