DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR […]
Tag: PCR
STEPS INVOLVED IN PERFORMING REAL-TIME PCR
Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, […]
INTRODUCTION TO REAL TIME POLYMERASE CHAIN REACTION (RT PCR)
The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or complementary DNA […]
GENOTYPIC DETECTION OF ANTIBIOTIC RESISTANT MICROBES
The genotypic detection and characterization of antibiotic resistant genes in pathogenic bacteria is more specific in characterizing resistance genes than the phenotypic detection methods. The […]
PCR TECHNIQUE
PCR is the acronym for “polymerase chain reaction”. Polymerase chain reaction is the molecular biology technique that is used to amplify or copy specific gene […]
THERMOCYCLER (PCR Machine)
Thermocycler or thermal cycler is a piece of equipment is used for the copying or amplification of specific sequences of nucleotides through a controlled timing […]