Gram staining

GRAM SMEAR OF CEREBROSPINAL FLUID (CSF) SAMPLE

AIM: To detect the presence of pus cells and bacteria in cerebrospinal fluid (CSF) specimen. MATERIAL/APPARATUS: CSF specimen, Gram staining reagents, microscope, glass slide, immersion oil, Bunsen burner. METHOD/PROCEDURE FOR CSF GRAM STAINING REPORTING OF THE RESULT Look for Gram negative intracellular diplococcic, Gram negative rods, Gram positive diplococci, and pus cells and report same. […]

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Microbe Lab, Techniques in Microbiology Lab, , ,

GRAM STAINING TECHNIQUE

Gram staining is a general purpose bacteriological identification technique used in the bacteriology section of the microbiology laboratory to identify and differentiate bacteria into two groups i.e. Gram-positive and Gram-negative. It was discovered by Christian Gram (1853-1938), a Danish scientist in 1884. Christian Gram showed that the cells of some bacteria could be easily decolorized

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Microbe Lab, Bacteriology, Techniques in Microbiology Lab, , , , , , , , , ,

HANS CHRISTIAN JOACHIM GRAM (1853-1938)

Hans Gram developed a microbiological technique which is still used today in clinical microbiology practice and bacteriology in particular for microbial identification and classification. His technique is generally used in microbiology for differentiating and classifying bacteria into two major types based on their reaction to certain stains or dye. The technique he developed is called

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General Microbiology, , ,