Escherichia coli is a facultative, enteric, Gram-negative, motile/flagellated, and lactose-fermenting rod that occur in the genus Escherichia and family Enterobacteria or Enterobacteriaceae. Enterobacteriaceae are bacteria that naturally exist in the intestinal tract of animals and humans, and also found in water and soil. Because the natural habitat of E. coli is the intestinal tract of […]
Triple sugar iron agar (TSIA) is a differential agar medium used to different lactose-fermenting Enterobacteriaceae from non-lactose fermenting enterobacteria. The medium usually contains three sugars viz: sucrose, glucose, and lactose, and phenol red as a pH indicator. TSIA is a synonymous biochemical test to Kliger’s iron agar (KIA) test. Both TSIA and KIA are used
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Biochemical Tests in Microbiology Lab
Indole test (Tryptophan hydrolysis) is used to identify Enterobacteria that breakdown tryptophan (an amino acid) to produce indole. The indole test is a biochemical test performed on bacterial species to determine the ability of the organism to convert tryptophan into the indole. Tryptophan hydrolysis is catalyzed by the enzyme tryptophanase to produce indole and pyruvic
