ADVANTAGES OF CELL CULTURE TECHNIQUES DISADVANTAGES OF CELL CULTURE TECHNIQUES References Alberts B, Bray D, Johnson A, Lewis J, Raff M, Roberts K andWalter P (1998). Essential Cell Biology: An Introduction to the Molecular Biology of the Cell. Third edition. Garland Publishing Inc., New York. Alberts B, Bray D, Lewis J, Raff M, Roberts K […]
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Cell Culture Notes
Cell culture technique is defined as the process by which prokaryotic, eukaryotic or plant cells are grown or cultured in vitro under controlled laboratory conditions. Cell culture techniques have applications in the following fields: DISEASE DIAGNOSIS Cell culture techniques are applied in clinical medicine for the diagnosis of infectious diseases especially diseases caused by pathogenic
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Cell Culture Notes
Laminar flow cabinet (hood) The laminar flow biological safety cabinet which can also be called hood or cell culture hood is one of the most important equipment in the cell (tissue) culture laboratory. This piece of equipment helps to provide sterile environment to work with cells without any fear of contamination. It is a filtration
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Cell Culture Notes
In the cell culture laboratory, there are many cell culture techniques that are routinely engaged or carried out. These various types of cell culture techniques are shown below: Their only advantage is that they may exhibit some physiological behaviour similar to that obtainable in vivo because they are freshly isolated cells. Primary cell cultures are
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Cell Culture NotesThe history of cell culture cannot be complete without the mention of Henrietta Lacks, whose immortal cell lines (HeLa cells) from cervical carcinoma helped revolutionize the field of cell culture. HeLa cells are immortal cell lines that was derived from a deadly cervical tumor from a cancer patient (named Henrietta Lacks) in 1951 after she
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Cell Culture Notes
Why is cell, tissue or organ culture embarked upon despite the ethical issues surrounding its development, acceptance and usage? Cell culture has application in a variety of biomedical sciences and even in the industry. It has become one of the major tools used in the life sciences world over. Its application has greatly revolutionized the
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Cell Culture Notes
The living cells that are cultured may include: When a whole organ or intact organ fragments of an organism (plant or animal) are removed from an organism and cultured in vitro for the purpose of studying their function and development, the process is called organ culture. The culturing of cells, tissues or organs occurs in
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Cell Culture Notes
Cell or tissue culture experimentations should not be carried out in the regular laboratory space where other laboratory investigations are undertaken. This is critical to avoid contamination of cells in the cell culture plates and also to ensure that all the physiochemical environmental factors that encourage optimal growth of the cells are provided. Thus, cell
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Cell Culture NotesCell culture is the laboratory technique of growing and maintaining the cells of multicellular organisms (plants and animal cells inclusive) in a favourable artificial environment conducive for growth. It is the maintenance of animal cells (inclusive of plant and human cell lines) in vitro.Such an artificial environment or growth medium mimics the internal environment of
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Cell Culture Notes