Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. By measuring the amount of amplified product at each stage during the PCR cycle, quantification is […]
STEPS INVOLVED IN PERFORMING REAL-TIME PCR Read More »
Molecular Microbiology, Techniques in Microbiology Lab
The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specific sequences within a DNA or complementary DNA (cDNA) template can be copied, or “amplified”, many thousand- to a million-fold using sequence-specific oligonucleotides (or primers), heat-stable DNA polymerase, and thermal cycling. In traditional (endpoint) PCR, detection and quantification
INTRODUCTION TO REAL TIME POLYMERASE CHAIN REACTION (RT PCR) Read More »
Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab