monoclonal antibody

SIGNIFICANCE OF MONOCLONAL AND POLYCLONAL ANTIBODIES

The two basic forms in which antibodies can be produced in purified forms in the laboratory are as monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies are homogenous antibodies produced in the laboratory by a single clone of cells (known as hybridoma cells), and all of which exhibit the same antigenic specificity. Hybridomas are cells capable […]

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Immunology & Immune System, ,

ANTIBODY SELECTION AND STAINING FOR IMMUNOHISTOCHEMISTRY

We have looked at deparaffinization and rehydration of paraffin-embedded tissue, as well as antigen-retrieval protocol and permeabilization and blocking non-specific binding on the sectioned tissue sample on the slide. In this section, we look briefly on antibody selection and antibody staining for immunohistochemistry. The selection of antibody for immunohistochemistry analysis is important for the overall

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Immunology & Immune System, , ,