Southern blotting, developed by Sir Edward M. Southern in 1975, is a molecular technique used to detect specific DNA sequences. It involves transferring DNA from a gel to a nitrocellulose membrane, followed by hybridization with radiolabeled probes. This method is pivotal in DNA analysis, forensic science, and paternity testing.
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Biotechnology, Molecular Microbiology
When phenol or a mixture of phenol:chloroform is mixed with a cell Lysate, two phases form: an aqueous and an organic phase. The polar DNA partitions into the polar or “aqueous phase”, and denatured proteins and other cellular debris partition into the organic phase. Salts are thought to dehydrate proteins, causing them to become less
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Biotechnology, Molecular Microbiology
The central framework of molecular biology otherwise known as the “central dogma” is the starting point for the actual course of movement of genetic information within the cell’s nucleus of an organism. The transfer of genetic information within the cell of an organism (i.e. in the nucleus) from deoxyribonucleic acid (DNA) to ribonucleic acid (RNA)
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Biotechnology, Molecular Microbiology
Genomic DNA is a double helix structure that is composed of several components including purines (adenine and guanine) and pyrimidines (cytosine and thymine) as nitrogenous bases, deoxyribose sugar (a pentose sugar) and phosphate groups. It is a large nucleic acid molecule composed mainly of two polynucleotide chains or strands that are coiled in a complementary
Born on the 6th of April, 1928, James Watson is an American molecular biologist, geneticist, and zoologist, who is best known as a co-discoverer of the structure of deoxyribonucleic acid (DNA) in 1953. Watson co-discovered the structure of the DNA with Francis Crick (an English molecular biologist, biophysicist, and neuroscientist) in 1953. Watson and Crick
Francis Crick was an English molecular biologist, biophysicist, and neuroscientist. He co-discovered the molecular structure of the genetic material (DNA) with James Watson in 1953. Francis Crick showed in his work that genetic information flows from the DNA to RNA and then to protein; and he referred to this very important concept of molecular biology
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General Microbiology