REAL-TIME PCR COMPONENTS

Real-time PCR primer design

Good primer design is one of the most important parameters in real-time PCR. This is why many researchers choose to purchase TaqMan® Assay products—primers and probes for real-time PCR designed using a proven algorithm and trusted by scientists around the world. If you choose to design your own real-time PCR primers, keep in mind that […]

Real-time PCR primer design Read More »

Biotechnology, Molecular Microbiology, Techniques in Microbiology Lab, , ,

REAL-TIME PCR COMPONENTS

DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product.

REAL-TIME PCR COMPONENTS Read More »

Molecular Microbiology, Techniques in Microbiology Lab, , , , , , , , , ,