WAYS TO SEPARATE DNA FROM OTHER CELLULAR MATERIALS: BINDING AND WASHING
When phenol or a mixture of phenol:chloroform is mixed with a cell Lysate, two phases form: an aqueous and an organic phase. The polar DNA partitions into the polar or “aqueous phase”, and denatured proteins and other cellular debris partition into the organic phase. Salts are thought to dehydrate proteins, causing them to become less […]
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Biotechnology, Molecular Microbiology