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Real-time PCR fluorescence detection systems

Real-time PCR fluorescence detection systems Real-time fluorescent PCR chemistries Many real-time fluorescent PCR chemistries exist, but the most widely used are 5” nuclease assays such as TaqMan®Assays and SYBR® Green dye–based assays (Figure 1). The 5” nuclease assay is named for the 5” nuclease activityassociated with Taq DNA polymerase (Figure 2). The 5” nuclease domain […]

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Biotechnology, Notes on Genetic Engineering & Recombinant DNA Technology, Techniques in Microbiology Lab3” nuclease assay specificity, 5” nuclease assay specificity, DNA polymerase, probe, real time PCR, Real-time PCR fluorescence detection systems, TaqMan, TaqMan probe, TaqMan® probe types

REAL-TIME PCR COMPONENTS

DrChika

DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product.

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Notes on Genetic Engineering & Recombinant DNA Technology, Techniques in Microbiology Labdeoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP), Deoxynucelotide triphosphates, deoxythymidine triphosphate (dTTP), DNA polymerase, dNTPs, PCR, REAL-TIME PCR COMPONENTS, Reverse transcriptase, Taq DNA polymerase

STEPS INVOLVED IN PERFORMING REAL-TIME PCR

DrChika

Real-time PCR is a variation of the standard PCR technique that is commonly used to quantify DNA or RNA in a sample. Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined. By measuring the amount of amplified product at each stage during the PCR cycle, quantification is

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Notes on Genetic Engineering & Recombinant DNA Technology, Techniques in Microbiology Labannealing, denaturation, DNA polymerase, extension, PCR, PCR reaction, primers, qRT-PCR, quantitative reverse transcriptase PCR, real time PCR, RT PCR, steps involved in real time PCR

HOW TO DESIGN PRIMERS FOR YOUR PCR EXPERIMENT

DrChika

Primers are short stretches of DNA that target unique sequences of a DNA molecule and help identify a unique part of a genome or a gene to be copied further. They are usually 18 to 25 nucleotides long. Primers can be synthesized and used for your PCR (polymerase chain reaction) and other molecular biology experiment

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Biotechnology, Microbe Lab, Notes on Genetic Engineering & Recombinant DNA Technology, Techniques in Microbiology LabDNA polymerase, HOW TO DESIGN PRIMERS FOR YOUR PCR EXPERIMENT, okazaki fragments, oligonucleotide primers, pcr experiment, PCR primers, primer, primer design, primers