DNA isolation

GENOMIC DNA ISOLATION

The Isolation of Genomic DNA from the bacterium, Escherichia coli is explained in this section Aim of the experiment: To isolate the genomic DNA from Escherichia coli DH5α cells. PRINCIPLE OF THE EXPERIMENT The isolation and purification of DNA from cells is one of the most common procedures in contemporary molecular biology and embodies a transition […]

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Biotechnology, Molecular Microbiology,

TYPES OF SOLID SUPPORT FOR BINDING DNA

The types of solid support for binding DNA are as follows: 1. Silica Matrix Silica Matrix is a solid support for binding DNA. DNA binds to silica in the presence of high concentrations of chaotropic salts (e.g., guanidium HCL), but protein does not. Salts can be removed with an alcohol-based wash and DNA eluted in

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Molecular Microbiology, , , ,