REAL-TIME PCR COMPONENTS
DNA polymerasePCR performance is often related to the thermostable DNA polymerase, so enzyme selection is critical to success. One of the main factors affecting PCR specificity is the fact that Taq DNA polymerase has residual activity at low temperatures. Primers can anneal nonspecifically to DNA during reaction setup, allowing the polymerase to synthesize nonspecific product. […]
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Notes on Genetic Engineering & Recombinant DNA Technology, Techniques in Microbiology Lab